Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects

Baknoon Ham, Hyung Bin Hwang, Sang Hoon Jung, Sungyul Chang, Kui Dong Kang, Man Jae Kwon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: The aim of this study was to identify differences in the major (core vs. variable) microbial genera of human subjects with and without diabetes. Methods: Bacterial 16S rRNA genes obtained from conjunctival swabs of 19 healthy subjects and 30 diabetic patients were sequenced using the Illumina MiSeq platform, and the sequencing data were analyzed using QIIME 1.9.1. To elucidate the microbial diversity in the ocular surface (OS), test programs from various bioinformatics domains were used. Results: Diversity index and rarefaction analysis showed that the microbial community of the diabetic patients was more diverse than that of the healthy subjects. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria and Bacteroidetes were the dominant taxa present in the OS, and there was a significant difference in the relative abundance of the bacterial phyla between the diabetic patients and control subjects. Proteobacteria were more abundant in the diabetic group, whereas Firmicutes was more abundant in the control group. Analysis of bacterial taxa at the genus level showed that the core microbiome of diabetic patients comprised Acinetobacter, Burkholderia, Sphingomonas, and Ralstonia, whereas that of the controls comprised Bradyrhizobiaceae, Staphylococcus, Corynebacterium, Pseudomonas, Novosphingobium, Neisseriaceae, and Acinetobacter. Conclusions: There was a significant difference in the microbial community composition between diabetic patients and healthy subjects. A high abundance of Acinetobacter in the OS of diabetic patients may arise from the unique characteristics of the OS compared with those of other organ surfaces.

Original languageEnglish
Pages (from-to)314-324
Number of pages11
JournalCurrent Eye Research
Volume43
Issue number3
DOIs
Publication statusPublished - 2018 Mar 4

Fingerprint

Healthy Volunteers
Acinetobacter
Proteobacteria
Bradyrhizobiaceae
Neisseriaceae
Ralstonia
Sphingomonas
Bacteroidetes
Burkholderia
Corynebacterium
Actinobacteria
Microbiota
Cyanobacteria
Pseudomonas
Computational Biology
Staphylococcus
rRNA Genes
Control Groups
Firmicutes

Keywords

  • Bacterial diversity
  • Bradyrhizobiaceae
  • Burkholderia
  • LFR analysis
  • Staphylococcus

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects. / Ham, Baknoon; Hwang, Hyung Bin; Jung, Sang Hoon; Chang, Sungyul; Kang, Kui Dong; Kwon, Man Jae.

In: Current Eye Research, Vol. 43, No. 3, 04.03.2018, p. 314-324.

Research output: Contribution to journalArticle

Ham, Baknoon ; Hwang, Hyung Bin ; Jung, Sang Hoon ; Chang, Sungyul ; Kang, Kui Dong ; Kwon, Man Jae. / Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects. In: Current Eye Research. 2018 ; Vol. 43, No. 3. pp. 314-324.
@article{2054154779254c6088b45e098cb32603,
title = "Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects",
abstract = "Purpose: The aim of this study was to identify differences in the major (core vs. variable) microbial genera of human subjects with and without diabetes. Methods: Bacterial 16S rRNA genes obtained from conjunctival swabs of 19 healthy subjects and 30 diabetic patients were sequenced using the Illumina MiSeq platform, and the sequencing data were analyzed using QIIME 1.9.1. To elucidate the microbial diversity in the ocular surface (OS), test programs from various bioinformatics domains were used. Results: Diversity index and rarefaction analysis showed that the microbial community of the diabetic patients was more diverse than that of the healthy subjects. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria and Bacteroidetes were the dominant taxa present in the OS, and there was a significant difference in the relative abundance of the bacterial phyla between the diabetic patients and control subjects. Proteobacteria were more abundant in the diabetic group, whereas Firmicutes was more abundant in the control group. Analysis of bacterial taxa at the genus level showed that the core microbiome of diabetic patients comprised Acinetobacter, Burkholderia, Sphingomonas, and Ralstonia, whereas that of the controls comprised Bradyrhizobiaceae, Staphylococcus, Corynebacterium, Pseudomonas, Novosphingobium, Neisseriaceae, and Acinetobacter. Conclusions: There was a significant difference in the microbial community composition between diabetic patients and healthy subjects. A high abundance of Acinetobacter in the OS of diabetic patients may arise from the unique characteristics of the OS compared with those of other organ surfaces.",
keywords = "Bacterial diversity, Bradyrhizobiaceae, Burkholderia, LFR analysis, Staphylococcus",
author = "Baknoon Ham and Hwang, {Hyung Bin} and Jung, {Sang Hoon} and Sungyul Chang and Kang, {Kui Dong} and Kwon, {Man Jae}",
year = "2018",
month = "3",
day = "4",
doi = "10.1080/02713683.2017.1406528",
language = "English",
volume = "43",
pages = "314--324",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects

AU - Ham, Baknoon

AU - Hwang, Hyung Bin

AU - Jung, Sang Hoon

AU - Chang, Sungyul

AU - Kang, Kui Dong

AU - Kwon, Man Jae

PY - 2018/3/4

Y1 - 2018/3/4

N2 - Purpose: The aim of this study was to identify differences in the major (core vs. variable) microbial genera of human subjects with and without diabetes. Methods: Bacterial 16S rRNA genes obtained from conjunctival swabs of 19 healthy subjects and 30 diabetic patients were sequenced using the Illumina MiSeq platform, and the sequencing data were analyzed using QIIME 1.9.1. To elucidate the microbial diversity in the ocular surface (OS), test programs from various bioinformatics domains were used. Results: Diversity index and rarefaction analysis showed that the microbial community of the diabetic patients was more diverse than that of the healthy subjects. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria and Bacteroidetes were the dominant taxa present in the OS, and there was a significant difference in the relative abundance of the bacterial phyla between the diabetic patients and control subjects. Proteobacteria were more abundant in the diabetic group, whereas Firmicutes was more abundant in the control group. Analysis of bacterial taxa at the genus level showed that the core microbiome of diabetic patients comprised Acinetobacter, Burkholderia, Sphingomonas, and Ralstonia, whereas that of the controls comprised Bradyrhizobiaceae, Staphylococcus, Corynebacterium, Pseudomonas, Novosphingobium, Neisseriaceae, and Acinetobacter. Conclusions: There was a significant difference in the microbial community composition between diabetic patients and healthy subjects. A high abundance of Acinetobacter in the OS of diabetic patients may arise from the unique characteristics of the OS compared with those of other organ surfaces.

AB - Purpose: The aim of this study was to identify differences in the major (core vs. variable) microbial genera of human subjects with and without diabetes. Methods: Bacterial 16S rRNA genes obtained from conjunctival swabs of 19 healthy subjects and 30 diabetic patients were sequenced using the Illumina MiSeq platform, and the sequencing data were analyzed using QIIME 1.9.1. To elucidate the microbial diversity in the ocular surface (OS), test programs from various bioinformatics domains were used. Results: Diversity index and rarefaction analysis showed that the microbial community of the diabetic patients was more diverse than that of the healthy subjects. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria and Bacteroidetes were the dominant taxa present in the OS, and there was a significant difference in the relative abundance of the bacterial phyla between the diabetic patients and control subjects. Proteobacteria were more abundant in the diabetic group, whereas Firmicutes was more abundant in the control group. Analysis of bacterial taxa at the genus level showed that the core microbiome of diabetic patients comprised Acinetobacter, Burkholderia, Sphingomonas, and Ralstonia, whereas that of the controls comprised Bradyrhizobiaceae, Staphylococcus, Corynebacterium, Pseudomonas, Novosphingobium, Neisseriaceae, and Acinetobacter. Conclusions: There was a significant difference in the microbial community composition between diabetic patients and healthy subjects. A high abundance of Acinetobacter in the OS of diabetic patients may arise from the unique characteristics of the OS compared with those of other organ surfaces.

KW - Bacterial diversity

KW - Bradyrhizobiaceae

KW - Burkholderia

KW - LFR analysis

KW - Staphylococcus

UR - http://www.scopus.com/inward/record.url?scp=85035134255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035134255&partnerID=8YFLogxK

U2 - 10.1080/02713683.2017.1406528

DO - 10.1080/02713683.2017.1406528

M3 - Article

C2 - 29172724

AN - SCOPUS:85035134255

VL - 43

SP - 314

EP - 324

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 3

ER -