Disulfide bond influence on protein structural dynamics probed with 2D-IR vibrational echo spectroscopy

Haruto Ishikawa, Seongheun Kim, Kyungwon Kwak, Keisuke Wakasugi, Michael D. Fayer

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Intramolecular disulfide bonds are understood to play a role in regulating protein stability and activity. Because disulfide bonds covalently link different components of a protein, they influence protein structure. However, the effects of disulfide bonds on fast (subpicosecond to ≈100 ps) protein equilibrium structural fluctuations have not been characterized experimentally. Here, ultrafast 2D-IR vibrational echo spectroscopy is used to examine the constraints an intramolecular disulfide bond places on the structural fluctuations of the protein neuroglobin (Ngb). Ngb is a globin family protein found in vertebrate brains that binds oxygen reversibly. Like myoglobin (Mb), Ngb has the classical globin fold and key residues around the heme are conserved. Furthermore, the heme-ligated CO vibrational spectra of Mb (Mb-CO) and Ngb (Ngb-CO) are virtually identical. However, in contrast to Mb, human Ngb has an intramolecular disulfide bond that affects its oxygen affinity and protein stability. By using 2D-IR vibrational echo spectroscopy, we investigated the equilibrium protein dynamics of Ngb-CO by observing the CO spectral diffusion (time dependence of the 2D-IR line shapes) with and without the disulfide bond. Despite the similarity of the linear FTIR spectra of Ngb-CO with and without the disulfide bond, 2D-IR measurements reveal that the equilibrium sampling of different protein configurations is accelerated by disruption of the disulfide bond. The observations indicate that the intramolecular disulfide bond in Ngb acts as an inhibitor of fast protein dynamics even though eliminating it does not produce significant conformational change in the protein's structure.

Original languageEnglish
Pages (from-to)19309-19314
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number49
DOIs
Publication statusPublished - 2007 Dec 4
Externally publishedYes

Fingerprint

Disulfides
Spectrum Analysis
Carbon Monoxide
Myoglobin
Proteins
Globins
Protein Stability
Heme
Oxygen
neuroglobin
Fourier Transform Infrared Spectroscopy
Vertebrates
Brain

Keywords

  • FTIR
  • Neuroglobin
  • Ultrafast 2D-IR

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Disulfide bond influence on protein structural dynamics probed with 2D-IR vibrational echo spectroscopy. / Ishikawa, Haruto; Kim, Seongheun; Kwak, Kyungwon; Wakasugi, Keisuke; Fayer, Michael D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 49, 04.12.2007, p. 19309-19314.

Research output: Contribution to journalArticle

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