Diversity and varietal classification of Hibiscus syriacus L. with the heterogeneity within retrotransposon-like elements

Seung Jac Lee, Ji Ung Jeung, Sung Ki Cho, Bo Young Um, Won Il Chung, Jung Myung Bae, Jeong Sheop Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Retrotransposons are present in multi-copy numbers that are integrated into plant genomes with considerable heterogeneous sequences within a single plant and between plant species, which allows the use of retrotransposons as additional sources of DNA polymorphism. A primer design for the sequence-tagged specific site and cleaved amplified polymorphic sequences (STS-CAPs) that are derived from retrotransposon-like sequences was developed for the molecular marker analysis in Hibiscus syriacus. This method was applied for the detection of sequence variations of intact retrotransposons that exist in plant genomes, which resulted in higher polymorphisms than in the amplified fragment length polymorphism (AFLP). Through STS-CAPs, specific fingerprinting data among H. syriacus varieties can be easily distinguished and generated with reproducible results. It could also be adapted to any species that possess multi-copy retrotransposons for varietal identification as well as the assessment of genetic relationships.

Original languageEnglish
Pages (from-to)362-368
Number of pages7
JournalMolecules and cells
Volume13
Issue number3
Publication statusPublished - 2002 Jun

Keywords

  • CAPs
  • Fingerprinting
  • Hibiscus syriacus
  • Polymorphism
  • Retrotransposon-like element
  • STS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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