DMSO effects on FRET to dye-labeled DNA in conjugated polymer-based DNA detection

Mijeong Kang, Boram Kim, Han Young Woo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Solvent effects were studied in fluorescence resonance energy transfer (FRET) from a cationic polyfluorene copolymer (FHQ, FPQ) to a fluorescein (Fl)-labeled oligonucleotide (ssDNA-Fl). Upon addition of dimethyl sulfoxide (DMSO), optical properties of the polymers and the probe dye were substantially modified. And the FRET-induced Fl emission was measured by directly exciting the polymer within the complex, polymer/ssDNA-Fl. The FRET signal was successfully modulated with changing the DMSO content. In the case of FHQ, the FRET-induced Fl emission was seriously quenched in phosphate buffer solution (PBS), while a salient FRET signal was observed in a 80 vol% DMSO/PBS mixture (36.8 time higher than that in PBS). The FPQ-sensitized FRET signal was also 3.8-fold amplified by the presence of DMSO. That result is from the decrease of hydrophobic interactions between the polymer and ssDNA-Fl, which induces the weaker polymer/ssDNA-Fl complexation with longer intermolecular separation. The gradual decrease in Fl PL quenching with increasing the DMSO content was investigated by measuring the Stern- Volmer quenching constants (3.3-4.2 × 10 6 M-1 in PBS, 0.56-1.1 x 106 M-1 in 80 vol% DMSO) in PBS/DMSO mixtures. The substantially reduced PL quenching would amplify the resulting FRET Fl signal. This approach suggests a simple way of modifying the fine-structure of polymer/ssDNA-Fl and improving the detection sensitivity in conjugated polymer-based FRET bioassays.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
DOIs
Publication statusPublished - 2011 Apr 1
Externally publishedYes
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII - San Francisco, CA, United States
Duration: 2011 Jan 242011 Jan 27

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7908
ISSN (Print)1605-7422

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
CountryUnited States
CitySan Francisco, CA
Period11/1/2411/1/27

Fingerprint

Fluorescence Resonance Energy Transfer
Dimethyl sulfoxide
Conjugated polymers
resonance fluorescence
Dimethyl Sulfoxide
Fluorescein
Polymers
DNA
Coloring Agents
Dyes
deoxyribonucleic acid
dyes
energy transfer
polymers
phosphates
Phosphates
buffers
Buffers
Quenching
quenching

Keywords

  • conjugated polymers (CPs)
  • dimethyl sulfoxide (DMSO)
  • DNA detection
  • Fluorescence resonance energy transfer (FRET)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kang, M., Kim, B., & Woo, H. Y. (2011). DMSO effects on FRET to dye-labeled DNA in conjugated polymer-based DNA detection. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII [790809] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7908). https://doi.org/10.1117/12.874398

DMSO effects on FRET to dye-labeled DNA in conjugated polymer-based DNA detection. / Kang, Mijeong; Kim, Boram; Woo, Han Young.

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII. 2011. 790809 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7908).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kang, M, Kim, B & Woo, HY 2011, DMSO effects on FRET to dye-labeled DNA in conjugated polymer-based DNA detection. in Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII., 790809, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, San Francisco, CA, United States, 11/1/24. https://doi.org/10.1117/12.874398
Kang M, Kim B, Woo HY. DMSO effects on FRET to dye-labeled DNA in conjugated polymer-based DNA detection. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII. 2011. 790809. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.874398
Kang, Mijeong ; Kim, Boram ; Woo, Han Young. / DMSO effects on FRET to dye-labeled DNA in conjugated polymer-based DNA detection. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII. 2011. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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abstract = "Solvent effects were studied in fluorescence resonance energy transfer (FRET) from a cationic polyfluorene copolymer (FHQ, FPQ) to a fluorescein (Fl)-labeled oligonucleotide (ssDNA-Fl). Upon addition of dimethyl sulfoxide (DMSO), optical properties of the polymers and the probe dye were substantially modified. And the FRET-induced Fl emission was measured by directly exciting the polymer within the complex, polymer/ssDNA-Fl. The FRET signal was successfully modulated with changing the DMSO content. In the case of FHQ, the FRET-induced Fl emission was seriously quenched in phosphate buffer solution (PBS), while a salient FRET signal was observed in a 80 vol{\%} DMSO/PBS mixture (36.8 time higher than that in PBS). The FPQ-sensitized FRET signal was also 3.8-fold amplified by the presence of DMSO. That result is from the decrease of hydrophobic interactions between the polymer and ssDNA-Fl, which induces the weaker polymer/ssDNA-Fl complexation with longer intermolecular separation. The gradual decrease in Fl PL quenching with increasing the DMSO content was investigated by measuring the Stern- Volmer quenching constants (3.3-4.2 × 10 6 M-1 in PBS, 0.56-1.1 x 106 M-1 in 80 vol{\%} DMSO) in PBS/DMSO mixtures. The substantially reduced PL quenching would amplify the resulting FRET Fl signal. This approach suggests a simple way of modifying the fine-structure of polymer/ssDNA-Fl and improving the detection sensitivity in conjugated polymer-based FRET bioassays.",
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AB - Solvent effects were studied in fluorescence resonance energy transfer (FRET) from a cationic polyfluorene copolymer (FHQ, FPQ) to a fluorescein (Fl)-labeled oligonucleotide (ssDNA-Fl). Upon addition of dimethyl sulfoxide (DMSO), optical properties of the polymers and the probe dye were substantially modified. And the FRET-induced Fl emission was measured by directly exciting the polymer within the complex, polymer/ssDNA-Fl. The FRET signal was successfully modulated with changing the DMSO content. In the case of FHQ, the FRET-induced Fl emission was seriously quenched in phosphate buffer solution (PBS), while a salient FRET signal was observed in a 80 vol% DMSO/PBS mixture (36.8 time higher than that in PBS). The FPQ-sensitized FRET signal was also 3.8-fold amplified by the presence of DMSO. That result is from the decrease of hydrophobic interactions between the polymer and ssDNA-Fl, which induces the weaker polymer/ssDNA-Fl complexation with longer intermolecular separation. The gradual decrease in Fl PL quenching with increasing the DMSO content was investigated by measuring the Stern- Volmer quenching constants (3.3-4.2 × 10 6 M-1 in PBS, 0.56-1.1 x 106 M-1 in 80 vol% DMSO) in PBS/DMSO mixtures. The substantially reduced PL quenching would amplify the resulting FRET Fl signal. This approach suggests a simple way of modifying the fine-structure of polymer/ssDNA-Fl and improving the detection sensitivity in conjugated polymer-based FRET bioassays.

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