DNA-based identification of South Korean Megaloptera larvae with taxonomic notes

Sang Woo Jung, Tatyana S. Vshivkova, Yeon Jae Bae

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mitochondrial DNA (mtDNA) sequences, which serve as DNA barcodes, have been used to associate immature and adult stages of insects and to delineate species. The partial mitochondrial cytochrome c oxidase subunit I (COI) gene sequences for South Korean Megaloptera (all known in the adult stage) were tested to identify undetermined larvae as a rapid and effective method from 31 specimens (16 adults and 15 larvae). The COI gene sequences distinguished all six known Megaloptera species, with a low genetic distance between larvae and adults (0.50±0.21%). Based on the COI gene sequences, we associated five types of larvae with known adults including the following four species of newly described larval stages: Sialis annae Vshivkova, 1979 (Megaloptera: Sialidae); Neochauliodes formosanus (Okamoto, 1910) (Megaloptera: Corydalidae); Parachauliodes asahinai Liu et al., 2008 (Megaloptera: Corydalidae); and Protohermes xanthodes Navás, 1913 (Megaloptera: Corydalidae). The known larval stage of Sialis koreana Jung and Bae, 2012 (Megaloptera: Sialidae) was confirmed, and the morphological variation in the male genitalia of Sialis longidens Klingstedt, 1932 (Megaloptera: Sialidae) is discussed. A larval key to the six South Korean species of Megaloptera is provided.

Original languageEnglish
Pages (from-to)123-139
Number of pages17
JournalCanadian Entomologist
Volume148
Issue number2
DOIs
Publication statusPublished - 2015 Jun 4

Fingerprint

Megaloptera
Larva
larva
DNA
larvae
Sialis
Sialidae
Corydalidae
Oxidoreductases
Male Genitalia
gene
Genes
nucleotide sequences
Electron Transport Complex IV
Mitochondrial DNA
Insects
mitochondrial DNA
cytochrome
insect
DNA barcoding

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Structural Biology

Cite this

DNA-based identification of South Korean Megaloptera larvae with taxonomic notes. / Jung, Sang Woo; Vshivkova, Tatyana S.; Bae, Yeon Jae.

In: Canadian Entomologist, Vol. 148, No. 2, 04.06.2015, p. 123-139.

Research output: Contribution to journalArticle

Jung, Sang Woo ; Vshivkova, Tatyana S. ; Bae, Yeon Jae. / DNA-based identification of South Korean Megaloptera larvae with taxonomic notes. In: Canadian Entomologist. 2015 ; Vol. 148, No. 2. pp. 123-139.
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