DNA damage in T and B lymphocytes, bone marrow, spleens, and livers of rats exposed to benzene

Eun Il Lee, Hosub Im, Eunha Oh, Woon W. Jung, Hyung S. Kang, Dong Geun Sul

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Single-cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T and B lymphocytes, spleens, bone marrow, and livers of rats exposed to benzene at a concentration of 100, 200, or 400 ppm for 2 or 4 wk. The level of t,t-muconic acid (t,t-MA), which is a urinary benzene metabolite, was determined. In the control rats, mean Olive tail moments in the T and B lymphocytes were 1.507 ± 0.398 and 1.579 ± 0.206, respectively. DNA damage in the T and B lymphocytes exposed to 400 ppm benzene for 4 wk caused those rats to exhibit the highest Olive tail moments, with their values measured as 4.351 ± 0.510 and 3.140 ± 0.631, respectively. Also, the t,t-MA levels increased directly with increasing benzene exposure time and dose during the 4 wk. After 4 wk, the levels of t,t-MA in urine from rats exposed to 100, 200, and 400 ppm were 19.30 ± 5.62, 30.36 ± 4.46, and 46.93 ± 9.10 mg/g creatinine. In conclusion, the present study demonstrates that benzene exposure results in significant DNA damage in the T and B lymphocytes, bone marrow, spleens, and livers of rats. DNA damage in the blood cells and organs was also discovered to vary directly with benzene exposure, in both a dose-dependent and time-dependent manner. In addition, a similar trend regarding DNA damage was found in the blood cells and organs, and evidenced a good association with the level of t,t-MA in the urine.

Original languageEnglish
Pages (from-to)401-408
Number of pages8
JournalInhalation Toxicology
Volume17
Issue number7-8
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

Lymphocytes
Benzene
Liver
DNA Damage
Rats
Bone
B-Lymphocytes
Spleen
Bone Marrow
T-Lymphocytes
DNA
Olea
Tail
Blood Cells
Blood
Cells
Rat control
Urine
Comet Assay
Metabolites

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

DNA damage in T and B lymphocytes, bone marrow, spleens, and livers of rats exposed to benzene. / Lee, Eun Il; Im, Hosub; Oh, Eunha; Jung, Woon W.; Kang, Hyung S.; Sul, Dong Geun.

In: Inhalation Toxicology, Vol. 17, No. 7-8, 01.06.2005, p. 401-408.

Research output: Contribution to journalArticle

Lee, Eun Il ; Im, Hosub ; Oh, Eunha ; Jung, Woon W. ; Kang, Hyung S. ; Sul, Dong Geun. / DNA damage in T and B lymphocytes, bone marrow, spleens, and livers of rats exposed to benzene. In: Inhalation Toxicology. 2005 ; Vol. 17, No. 7-8. pp. 401-408.
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