DNA-enrichment microfluidic chip for chromatin immunoprecipitation

Hyun Jik Oh, Joong Yull Park, Sung Eun Park, Bo Yun Lee, Jong Sung Park, Suel Kee Kim, Tae Joong Yoon, Sang Hoon Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting association of individual proteins with specific genomic regions; the technique requires several complex steps and is tedious. In this paper, we develop a microbead-packed microfluidic chip which eliminates most of the laborious, timeconsuming, and skill-dependent processes of the ChIP procedure. A computational fluid dynamics model was established to analyze fluidic behavior in a microbeadpacked microchannel. With the use of the new chip, a ChIP procedure was performed to purify the GAPDH (glyceraldehyde 3-phosphate dehydrogenase) gene from human embryonic kidney cells (cell line 293). The ChIP capability of the microfluidic chip was evaluated and compared with that of a commercial assay kit; the precipitation performance of both methods was almost identical as shown by quantitative measurement of DNA. However, our chip offers the advantage of low resin volume, and the experimental time is greatly reduced. In addition, our method is less dependent on individual technical skill. Therefore, we expect that our microfluidic chip-based ChIP method will be widely used in DNA-, gene-, and protein-related research and will improve experimental efficiency.

Original languageEnglish
Pages (from-to)2832-2839
Number of pages8
JournalAnalytical Chemistry
Volume81
Issue number8
DOIs
Publication statusPublished - 2009 Apr 15

Fingerprint

Microfluidics
Chromatin
DNA
Glyceraldehyde-3-Phosphate Dehydrogenases
Fluidics
Microchannels
Dynamic models
Assays
Computational fluid dynamics
Proteins
Resins
Genes
Cells
Association reactions

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Oh, H. J., Park, J. Y., Park, S. E., Lee, B. Y., Park, J. S., Kim, S. K., ... Lee, S. H. (2009). DNA-enrichment microfluidic chip for chromatin immunoprecipitation. Analytical Chemistry, 81(8), 2832-2839. https://doi.org/10.1021/ac802034s

DNA-enrichment microfluidic chip for chromatin immunoprecipitation. / Oh, Hyun Jik; Park, Joong Yull; Park, Sung Eun; Lee, Bo Yun; Park, Jong Sung; Kim, Suel Kee; Yoon, Tae Joong; Lee, Sang Hoon.

In: Analytical Chemistry, Vol. 81, No. 8, 15.04.2009, p. 2832-2839.

Research output: Contribution to journalArticle

Oh, HJ, Park, JY, Park, SE, Lee, BY, Park, JS, Kim, SK, Yoon, TJ & Lee, SH 2009, 'DNA-enrichment microfluidic chip for chromatin immunoprecipitation', Analytical Chemistry, vol. 81, no. 8, pp. 2832-2839. https://doi.org/10.1021/ac802034s
Oh HJ, Park JY, Park SE, Lee BY, Park JS, Kim SK et al. DNA-enrichment microfluidic chip for chromatin immunoprecipitation. Analytical Chemistry. 2009 Apr 15;81(8):2832-2839. https://doi.org/10.1021/ac802034s
Oh, Hyun Jik ; Park, Joong Yull ; Park, Sung Eun ; Lee, Bo Yun ; Park, Jong Sung ; Kim, Suel Kee ; Yoon, Tae Joong ; Lee, Sang Hoon. / DNA-enrichment microfluidic chip for chromatin immunoprecipitation. In: Analytical Chemistry. 2009 ; Vol. 81, No. 8. pp. 2832-2839.
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