DNA hybridization electrochemical sensor using conducting polymer

Junhoe Cha, Jung Im Han, Young Choi, Dae Sung Yoon, Kwang W. Oh, Geunbae Lim

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

We report the use of poly(thiophen-3-yl-acetic acid 1,3-dioxo-1,3-dihydro-isoindol-2-yl ester (PTAE) for application to electrochemical hybridization sensor. A synthetic route for the thiophen-3-yl-acetic acid 1,3-dioxo-1,3-dihydro-isoindol-2-yl ester (TAE) is described, which is used as a monomer of conducting polymer sensor. A direct chemical substitution of probe oligonucleotide to good leaving group site in the PTAE is carried out on the conducting polymer film. A biological recognition can be monitored by comparison with the electrochemical signal (cyclic voltammogram) of single and double strand state oligonucleotide. The sensitivity of the electrochemical sensor is 0.62 μA/nmole and the detection limit is 1 nmole. The oxidation current of double strand state oligonucleotide is a half of that of single strand, that is corresponding to the decrease of electrochemical activity of conducting polymer with increase of stiffness of side group of the polymer. The oxidation current decreasing ratios of perfect matched and single nucleotide mismatched samples are 52 and 25-30%, respectively. The more decreasing ratio is attributable to the more steric hindrance of single nucleotide mismatched sample.

Original languageEnglish
Pages (from-to)1241-1247
Number of pages7
JournalBiosensors and Bioelectronics
Volume18
Issue number10
DOIs
Publication statusPublished - 2003 Sep
Externally publishedYes

Keywords

  • Conducting polymer
  • Electrochemical hybridization sensor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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