DNA methylome analysis of saturated aliphatic aldehydes in pulmonary toxicity

Yoon Cho, Mi Kyung Song, Tae Sung Kim, Jae Chun Ryu

Research output: Contribution to journalArticle

Abstract

Recent studies have investigated the epigenetic effects of environmental exposure to chemicals on human health. The associations of DNA methylation, environmental exposure and human diseases have been widely demonstrated. However, the use of gene methylation patterns as a predictive biomarker for exposure to environmental toxicants is relatively poorly understood. Here, we focused on low-molecular-weight saturated aliphatic aldehydes (LSAAs), which are important environmental risk factors in humans as major indoor air pollutants. Based on DNA methylation profiling in gene promoter regions, we analysed DNA methylation profiles following exposure of A549 cells to seven LSAAs (propanal, butanal, pentanal, hexanal, heptanal, octanal, and nonanal) to identify LSAA-characterized methylated sites and target genes, as well as to investigate whether exposure to LSAAs contributes to inducing of pulmonary toxicity. Additionally, by integrating DNA methylation and mRNA expression profile analyses, we identified core anti-correlated target genes. Gene ontology analysis of these target genes revealed several key biological processes. These findings suggest that alterations in DNA methylation by exposure to LSAAs provide novel epigenetic biomarkers for risk assessments. This DNA methylation-mRNA approach also reveals potential new mechanistic insights into the epigenetic actions of pulmonary toxicity.

Original languageEnglish
Article number10497
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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DNA Methylation
Aldehydes
Molecular Weight
Lung
DNA
Environmental Exposure
Epigenomics
Genes
Biomarkers
Biological Phenomena
Air Pollutants
Messenger RNA
Gene Ontology
DNA Fingerprinting
Genetic Promoter Regions
Methylation
Health

ASJC Scopus subject areas

  • General

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DNA methylome analysis of saturated aliphatic aldehydes in pulmonary toxicity. / Cho, Yoon; Song, Mi Kyung; Kim, Tae Sung; Ryu, Jae Chun.

In: Scientific Reports, Vol. 8, No. 1, 10497, 01.12.2018.

Research output: Contribution to journalArticle

Cho, Yoon ; Song, Mi Kyung ; Kim, Tae Sung ; Ryu, Jae Chun. / DNA methylome analysis of saturated aliphatic aldehydes in pulmonary toxicity. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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