Abstract
Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.
| Original language | English |
|---|---|
| Pages (from-to) | 444-450 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 10 |
| Issue number | 5 |
| Publication status | Published - 2005 Sep 1 |
Fingerprint
Keywords
- Epidermal growth factor (EGF)
- Expression profiling
- Immediate response
- Oligonucleotide microarray
- Schwannoma cell line
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Biomedical Engineering
Cite this
DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells. / Oh, Min-Kyu; Scoles, Daniels R.; Pulst, Stefan M.
In: Biotechnology and Bioprocess Engineering, Vol. 10, No. 5, 01.09.2005, p. 444-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells
AU - Oh, Min-Kyu
AU - Scoles, Daniels R.
AU - Pulst, Stefan M.
PY - 2005/9/1
Y1 - 2005/9/1
N2 - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.
AB - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.
KW - Epidermal growth factor (EGF)
KW - Expression profiling
KW - Immediate response
KW - Oligonucleotide microarray
KW - Schwannoma cell line
UR - http://www.scopus.com/inward/record.url?scp=27744443635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27744443635&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:27744443635
VL - 10
SP - 444
EP - 450
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
SN - 1226-8372
IS - 5
ER -