DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells

Min-Kyu Oh, Daniels R. Scoles, Stefan M. Pulst

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.

Original languageEnglish
Pages (from-to)444-450
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume10
Issue number5
Publication statusPublished - 2005 Sep 1

    Fingerprint

Keywords

  • Epidermal growth factor (EGF)
  • Expression profiling
  • Immediate response
  • Oligonucleotide microarray
  • Schwannoma cell line

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this