TY - JOUR
T1 - DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells
AU - Oh, Min Kyu
AU - Scoles, Daniels R.
AU - Pulst, Stefan M.
N1 - Funding Information:
^ÅâåçïäÉÇÖÉãÉåíë The authors thank Cedars Sinai Medical Center microarray core facility for performing the microar-ray experiment. This work was supported by the Korea Research Foundation, KRF-2004-005-D00057, and the Carmen and Louis Warschaw endowment for neurology, grants DAMD 17-99-1-9548 and 01-01-0703 from the USAMRAA, and grant R01 NS37883.
PY - 2005
Y1 - 2005
N2 - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.
AB - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.
KW - Epidermal growth factor (EGF)
KW - Expression profiling
KW - Immediate response
KW - Oligonucleotide microarray
KW - Schwannoma cell line
UR - http://www.scopus.com/inward/record.url?scp=27744443635&partnerID=8YFLogxK
U2 - 10.1007/BF02989827
DO - 10.1007/BF02989827
M3 - Article
AN - SCOPUS:27744443635
VL - 10
SP - 444
EP - 450
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
SN - 1226-8372
IS - 5
ER -