TY - JOUR
T1 - DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC)
AU - Baik, Seung Ho
AU - Jee, Bo Keun
AU - Choi, Jin Soo
AU - Yoon, Hyoung Kyu
AU - Lee, Kweon Haeng
AU - Kim, Yeul Hong
AU - Lim, Young
N1 - Funding Information:
Acknowledgments This work was supported by Grant (01-PJ3-PG6-01GN07-0004), Good Health R&D Project, Ministry of Health Welfare, Republic of Korea and by a grant of the Korea Health 21 R&D Project, Ministry of Health Welfare, Republic of Korea, (00-PJ3-PG6-GN02-0002).
PY - 2009/9
Y1 - 2009/9
N2 - Lung tumor cell DNA copy number alteration (CNA) was expected to display specific patterns such as a large-scale amplification or deletion of chromosomal arms, as previously published data have reported. Peripheral blood mononuclear cell (PBMC) CNA however, was expected to show normal variations in cancer patients as well as healthy individuals, and has thus been used as normal control DNA samples in various published studies. We performed array CGH to measure and compare genetic changes in terms of the CNA of PBMC samples as well as DNA isolated from tumor tissue samples, obtained from 24 non-small cell lung cancer patients. Contradictory to expectations, our studies showed that the PBMC CNA also showed chromosomal variant regions. The list included well-known tumor-associated NTRK1, FGF8, TP53, and TGFβ1 genes and potentially novel oncogenes such as THPO (3q27.1), JMJD1B, and EGR1 (5q31.2), which was investigated in this study. The results of this study highlighted the connection between PBMC and tumor cell genomic DNA in lung cancer patients. However, the application of these studies to cancer prognosis may pose a challenge due to the large amount of information contained in genetic predisposition and family history that has to be processed for useful downstream clinical applications.
AB - Lung tumor cell DNA copy number alteration (CNA) was expected to display specific patterns such as a large-scale amplification or deletion of chromosomal arms, as previously published data have reported. Peripheral blood mononuclear cell (PBMC) CNA however, was expected to show normal variations in cancer patients as well as healthy individuals, and has thus been used as normal control DNA samples in various published studies. We performed array CGH to measure and compare genetic changes in terms of the CNA of PBMC samples as well as DNA isolated from tumor tissue samples, obtained from 24 non-small cell lung cancer patients. Contradictory to expectations, our studies showed that the PBMC CNA also showed chromosomal variant regions. The list included well-known tumor-associated NTRK1, FGF8, TP53, and TGFβ1 genes and potentially novel oncogenes such as THPO (3q27.1), JMJD1B, and EGR1 (5q31.2), which was investigated in this study. The results of this study highlighted the connection between PBMC and tumor cell genomic DNA in lung cancer patients. However, the application of these studies to cancer prognosis may pose a challenge due to the large amount of information contained in genetic predisposition and family history that has to be processed for useful downstream clinical applications.
KW - Array CGH
KW - Copy number alteration
KW - Non-small cell lung cancer
KW - Peripheral blood mononuclear cell
UR - http://www.scopus.com/inward/record.url?scp=68149125221&partnerID=8YFLogxK
U2 - 10.1007/s11033-008-9380-7
DO - 10.1007/s11033-008-9380-7
M3 - Article
C2 - 18975135
AN - SCOPUS:68149125221
SN - 0301-4851
VL - 36
SP - 1767
EP - 1778
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 7
ER -
