DNA repair activity of human rpS3 is operative to genotoxic damage in bacteria

Chang Young Jang, Jae Yung Lee, Joon Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human ribosomal protein S3 (rpS3), which has a DNA repair endonuclease activity, is a multifunctional protein. This protein is involved in DNA repair, translation, and apoptosis. In particular, rpS3 has a lyase activity, which cleaves the phosphodiester bond of damaged sites such as cyclobutane pyrimidine dimers and AP sites. Here, using deletion analysis, we identified that the repair endonuclease domain resides in the C-terminal region (165-243 aa) of rpS3. We also found that ectopic expression of GST-rpS3 in bacterial strain BL21 promoted the resistance of these cells to ultraviolet (UV) radiation and hydrogen peroxide (H2O2) treatment. The repair domain of rpS3 was sufficient to exhibit the resistance to UV irradiation and recover cell growth and viability, showing that the repair activity of rpS3 is responsible for the resistance to UV irradiation. Our study suggests that rpS3 is able to process DNA damage in bacteria via its repair domain, showing the resistance to genotoxic stress. This implies that rpS3-like activity could be operative in bacteria.

Original languageEnglish
Pages (from-to)484-490
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number3
Publication statusPublished - 2005 Jun 1

Fingerprint

DNA Repair
Bacteria
DNA
Repair
Proteins
Endonucleases
DNA Damage
Irradiation
Pyrimidine Dimers
Lyases
Deoxyribonuclease I
Cell growth
human RPS3 protein
ribosomal protein S3
Ultraviolet radiation
Hydrogen Peroxide
Cell Survival
Cell death
Radiation
Apoptosis

Keywords

  • DNA repair
  • Endonuclease
  • Repair domain
  • rpS3
  • UV resistance

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

DNA repair activity of human rpS3 is operative to genotoxic damage in bacteria. / Jang, Chang Young; Lee, Jae Yung; Kim, Joon.

In: Journal of Microbiology and Biotechnology, Vol. 15, No. 3, 01.06.2005, p. 484-490.

Research output: Contribution to journalArticle

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