DNA-templated silver nanoclusters as label-free, sensitive detection probes for potassium ions and nitric oxide

Jihyun Lee, Junghong Park, Hong Hee Lee, Hugh I. Kim, Won Jong Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

DNA-templated silver nanoclusters (DNA-AgNCs) have emerged as promising materials for sensing, bio-labelling, and bio-imaging due to their fluorogenic properties. Using the 12 mer DNA with a cytosine-rich sequence, AgCNs have been formed successfully. Herein, we develop two different types of DNA-AgNC systems for the detection of potassium ions (K + ) and nitric oxide (NO) by utilizing the structural change of DNA or DNA template transformation. In K + detection, a thrombin binding aptamer (TBA) was utilized as both the AgNC template and the K + binding probe. The fluorescence emission of the DNA1-AgNC probe was diminished proportionally to the amount of K + detected, which transforms the AgNC-adapted structure into G-quadruplex. On the other hand, NO detection has been accomplished by NO-induced deamination of cytosine. The structural change of cytosine to deoxyuridine destabilizes the DNA2-AgNC structure, resulting in quenching of fluorescence intensity. The characteristics of these two highly quantitative detection probes were analysed by UV absorption, fluorescence emission, and transmission electron microscopy (TEM). Each system provides a detection limit of 6.2 μM for K + and 0.1 μM for NO. We expect that these systems enable the opening of a new horizon for highly sensitive and selective detection systems. This journal is

Original languageEnglish
Pages (from-to)2616-2621
Number of pages6
JournalJournal of Materials Chemistry B
Volume2
Issue number17
DOIs
Publication statusPublished - 2014 May 7
Externally publishedYes

Fingerprint

Nanoclusters
Nitric oxide
Silver
Potassium
Labels
Nitric Oxide
DNA
Ions
Cytosine
Fluorescence
G-Quadruplexes
Deoxyuridine
Deamination
Transmission Electron Microscopy
Labeling
Limit of Detection
potassium oxide
Quenching
Transmission electron microscopy
Imaging techniques

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

DNA-templated silver nanoclusters as label-free, sensitive detection probes for potassium ions and nitric oxide. / Lee, Jihyun; Park, Junghong; Lee, Hong Hee; Kim, Hugh I.; Kim, Won Jong.

In: Journal of Materials Chemistry B, Vol. 2, No. 17, 07.05.2014, p. 2616-2621.

Research output: Contribution to journalArticle

Lee, Jihyun ; Park, Junghong ; Lee, Hong Hee ; Kim, Hugh I. ; Kim, Won Jong. / DNA-templated silver nanoclusters as label-free, sensitive detection probes for potassium ions and nitric oxide. In: Journal of Materials Chemistry B. 2014 ; Vol. 2, No. 17. pp. 2616-2621.
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