Does a growing tumour volume induce lymphangiogenesis? A study of oral/oropharyngeal cancer

Eun Jae Chung, Young Soo Rho, Seung-Kuk Baek, Jeong-Soo Woo, Soon Young Kwon, Nam-Joon Lee, Yang Seok Chae, Kwang-Yoon Jung

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2 Citations (Scopus)

Abstract

Objective: Tumour cell metastasis to regional lymph nodes is an early event in the spread of metastatic tumour. We postulated that an increased primary tumour volume (PTV) may modulate cervical nodal metastasis by altering lymphangiogenesis. Setting: We investigated 48 patients who had previously been diagnosed with cancer of the oral cavity and oropharynx. Methods: The tumour area was manually outlined from an axial magnetic resonance imaging series. The three-dimensional reconstruction software automatically calculated the PTV. Immunohistochemical staining was performed with vascular endothelial growth factor (VEGF)-C, VEGF-D, and D2-40 monoclonal antibodies on the paraffin-embedded tissues obtained from the primary tumour. Main Outcome Measures: The associations among the semiquantitative scores of the VEGF-C/D stained cancer cells, lymphatic vessel density (LVD), and PTV were investigated. Results: PTV had a significant relationship with LVD (p = .037) and N+ disease (p = .024). VEGF-C expression was significantly associated with lymph node metastasis (p = .018), increased LVD (p < .001), and tumour differentiation (p = .015). However, we found no significant relationship between VEGF-C expression and the PTV. Similarly, among the various clinical factors, we found that the N stage (p = .001) and LVD (p = .008) were significantly associated with VEGF-D expression. However there was no association between VEGF-D expression and the primary PTV. Conclusions: Although PTV had a significant relationship with LVD and N+ disease, we could not find any clear correlation between the expression of VEGF-C/D and the PTV.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalJournal of Otolaryngology - Head and Neck Surgery
Volume40
Issue number4
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Lymphangiogenesis
Oropharyngeal Neoplasms
Mouth Neoplasms
Tumor Burden
Vascular Endothelial Growth Factor D
Vascular Endothelial Growth Factor C
Lymphatic Vessels
Neoplasms
Neoplasm Metastasis
Lymph Nodes
Paraffin
Mouth
Software
Magnetic Resonance Imaging
Outcome Assessment (Health Care)
Staining and Labeling

Keywords

  • Lymphangiogenesis
  • Lymphatic metastasis
  • Oral cavity neoplasm
  • Oropharyngeal neoplasm
  • Tumour volume

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

@article{fcb2809c9ca84224ad1f0d2284b754c3,
title = "Does a growing tumour volume induce lymphangiogenesis? A study of oral/oropharyngeal cancer",
abstract = "Objective: Tumour cell metastasis to regional lymph nodes is an early event in the spread of metastatic tumour. We postulated that an increased primary tumour volume (PTV) may modulate cervical nodal metastasis by altering lymphangiogenesis. Setting: We investigated 48 patients who had previously been diagnosed with cancer of the oral cavity and oropharynx. Methods: The tumour area was manually outlined from an axial magnetic resonance imaging series. The three-dimensional reconstruction software automatically calculated the PTV. Immunohistochemical staining was performed with vascular endothelial growth factor (VEGF)-C, VEGF-D, and D2-40 monoclonal antibodies on the paraffin-embedded tissues obtained from the primary tumour. Main Outcome Measures: The associations among the semiquantitative scores of the VEGF-C/D stained cancer cells, lymphatic vessel density (LVD), and PTV were investigated. Results: PTV had a significant relationship with LVD (p = .037) and N+ disease (p = .024). VEGF-C expression was significantly associated with lymph node metastasis (p = .018), increased LVD (p < .001), and tumour differentiation (p = .015). However, we found no significant relationship between VEGF-C expression and the PTV. Similarly, among the various clinical factors, we found that the N stage (p = .001) and LVD (p = .008) were significantly associated with VEGF-D expression. However there was no association between VEGF-D expression and the primary PTV. Conclusions: Although PTV had a significant relationship with LVD and N+ disease, we could not find any clear correlation between the expression of VEGF-C/D and the PTV.",
keywords = "Lymphangiogenesis, Lymphatic metastasis, Oral cavity neoplasm, Oropharyngeal neoplasm, Tumour volume",
author = "Chung, {Eun Jae} and Rho, {Young Soo} and Seung-Kuk Baek and Jeong-Soo Woo and Kwon, {Soon Young} and Nam-Joon Lee and Chae, {Yang Seok} and Kwang-Yoon Jung",
year = "2011",
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language = "English",
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pages = "311--317",
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TY - JOUR

T1 - Does a growing tumour volume induce lymphangiogenesis? A study of oral/oropharyngeal cancer

AU - Chung, Eun Jae

AU - Rho, Young Soo

AU - Baek, Seung-Kuk

AU - Woo, Jeong-Soo

AU - Kwon, Soon Young

AU - Lee, Nam-Joon

AU - Chae, Yang Seok

AU - Jung, Kwang-Yoon

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Objective: Tumour cell metastasis to regional lymph nodes is an early event in the spread of metastatic tumour. We postulated that an increased primary tumour volume (PTV) may modulate cervical nodal metastasis by altering lymphangiogenesis. Setting: We investigated 48 patients who had previously been diagnosed with cancer of the oral cavity and oropharynx. Methods: The tumour area was manually outlined from an axial magnetic resonance imaging series. The three-dimensional reconstruction software automatically calculated the PTV. Immunohistochemical staining was performed with vascular endothelial growth factor (VEGF)-C, VEGF-D, and D2-40 monoclonal antibodies on the paraffin-embedded tissues obtained from the primary tumour. Main Outcome Measures: The associations among the semiquantitative scores of the VEGF-C/D stained cancer cells, lymphatic vessel density (LVD), and PTV were investigated. Results: PTV had a significant relationship with LVD (p = .037) and N+ disease (p = .024). VEGF-C expression was significantly associated with lymph node metastasis (p = .018), increased LVD (p < .001), and tumour differentiation (p = .015). However, we found no significant relationship between VEGF-C expression and the PTV. Similarly, among the various clinical factors, we found that the N stage (p = .001) and LVD (p = .008) were significantly associated with VEGF-D expression. However there was no association between VEGF-D expression and the primary PTV. Conclusions: Although PTV had a significant relationship with LVD and N+ disease, we could not find any clear correlation between the expression of VEGF-C/D and the PTV.

AB - Objective: Tumour cell metastasis to regional lymph nodes is an early event in the spread of metastatic tumour. We postulated that an increased primary tumour volume (PTV) may modulate cervical nodal metastasis by altering lymphangiogenesis. Setting: We investigated 48 patients who had previously been diagnosed with cancer of the oral cavity and oropharynx. Methods: The tumour area was manually outlined from an axial magnetic resonance imaging series. The three-dimensional reconstruction software automatically calculated the PTV. Immunohistochemical staining was performed with vascular endothelial growth factor (VEGF)-C, VEGF-D, and D2-40 monoclonal antibodies on the paraffin-embedded tissues obtained from the primary tumour. Main Outcome Measures: The associations among the semiquantitative scores of the VEGF-C/D stained cancer cells, lymphatic vessel density (LVD), and PTV were investigated. Results: PTV had a significant relationship with LVD (p = .037) and N+ disease (p = .024). VEGF-C expression was significantly associated with lymph node metastasis (p = .018), increased LVD (p < .001), and tumour differentiation (p = .015). However, we found no significant relationship between VEGF-C expression and the PTV. Similarly, among the various clinical factors, we found that the N stage (p = .001) and LVD (p = .008) were significantly associated with VEGF-D expression. However there was no association between VEGF-D expression and the primary PTV. Conclusions: Although PTV had a significant relationship with LVD and N+ disease, we could not find any clear correlation between the expression of VEGF-C/D and the PTV.

KW - Lymphangiogenesis

KW - Lymphatic metastasis

KW - Oral cavity neoplasm

KW - Oropharyngeal neoplasm

KW - Tumour volume

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EP - 317

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SN - 1916-0208

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