Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

Yoon Hee Cho, Dae-Sung Kim, Peter Geon Kim, Yong Sup Hwang, Myung Soo Cho, Shin Yong Moon, Dong Wook Kim, Jin Woo Chang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

Original languageEnglish
Pages (from-to)6-12
Number of pages7
JournalBiochemical and biophysical research communications
Volume341
Issue number1
DOIs
Publication statusPublished - 2006 Mar 3
Externally publishedYes

Fingerprint

Dopaminergic Neurons
Embryonic Stem Cells
Stem cells
Neurons
Rats
Dopamine
Parkinson Disease
Recovery
Subthalamic Nucleus
Animals
Cell Line
Brain

Keywords

  • Behavior recovery
  • Differentiation
  • Dopamine neurons
  • ES cells
  • Parkinson's disease

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model. / Cho, Yoon Hee; Kim, Dae-Sung; Kim, Peter Geon; Hwang, Yong Sup; Cho, Myung Soo; Moon, Shin Yong; Kim, Dong Wook; Chang, Jin Woo.

In: Biochemical and biophysical research communications, Vol. 341, No. 1, 03.03.2006, p. 6-12.

Research output: Contribution to journalArticle

Cho, Yoon Hee ; Kim, Dae-Sung ; Kim, Peter Geon ; Hwang, Yong Sup ; Cho, Myung Soo ; Moon, Shin Yong ; Kim, Dong Wook ; Chang, Jin Woo. / Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model. In: Biochemical and biophysical research communications. 2006 ; Vol. 341, No. 1. pp. 6-12.
@article{8f1b90a62bf44e66b172a7138e50bdfc,
title = "Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model",
abstract = "To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90{\%} of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with na{\"i}ve D3-derived cells (∼28{\%} DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.",
keywords = "Behavior recovery, Differentiation, Dopamine neurons, ES cells, Parkinson's disease",
author = "Cho, {Yoon Hee} and Dae-Sung Kim and Kim, {Peter Geon} and Hwang, {Yong Sup} and Cho, {Myung Soo} and Moon, {Shin Yong} and Kim, {Dong Wook} and Chang, {Jin Woo}",
year = "2006",
month = "3",
day = "3",
doi = "10.1016/j.bbrc.2005.12.140",
language = "English",
volume = "341",
pages = "6--12",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

AU - Cho, Yoon Hee

AU - Kim, Dae-Sung

AU - Kim, Peter Geon

AU - Hwang, Yong Sup

AU - Cho, Myung Soo

AU - Moon, Shin Yong

AU - Kim, Dong Wook

AU - Chang, Jin Woo

PY - 2006/3/3

Y1 - 2006/3/3

N2 - To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

AB - To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

KW - Behavior recovery

KW - Differentiation

KW - Dopamine neurons

KW - ES cells

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=30944461146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30944461146&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.12.140

DO - 10.1016/j.bbrc.2005.12.140

M3 - Article

VL - 341

SP - 6

EP - 12

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -