Downregulation of CHIP promotes ovarian cancer metastasis by inducing Snail-mediated epithelial–mesenchymal transition

Sun Mi Park, Seung Ho Park, Ki Jun Ryu, In Kyu Kim, Hyeontak Han, Hyo Jin Kim, Seon Hee Kim, Keun Seok Hong, Hyemin Kim, Minju Kim, Bok Im Cho, Jeong Doo Heo, Na Hyun Kim, Eun Mi Hwang, Jae-Yong Park, Jong In Yook, Hee Jun Cho, Cheol Hwangbo, Kwang Dong Kim, Hoseok SongJiyun Yoo

Research output: Contribution to journalArticle

Abstract

The epithelial–mesenchymal transition (EMT) plays a pivotal role in the conversion of early-stage tumors into invasive malignancies. The transcription factor Snail, an extremely unstable protein whose subcellular levels are regulated by many E3 ubiquitin ligases, promotes EMT as well as associated pathological characteristics including migration, invasion, and metastasis. Through yeast two-hybrid screening, we identified the carboxyl terminus of Hsc70-interacting protein (CHIP) as a novel Snail ubiquitin ligase that interacts with Snail to induce ubiquitin-mediated proteasomal degradation. Inhibition of CHIP expression increases Snail protein levels, induces EMT, and enhances in vitro migration and invasion as well as in vivo metastasis of ovarian cancer cells. In turn, Snail depletion abrogates all phenomena induced by CHIP depletion. Finally, Snail and CHIP expression is inversely correlated in ovarian tumor tissues. These findings establish the CHIP–Snail axis as a post-translational mechanism of EMT and cancer metastasis regulation.

Original languageEnglish
Pages (from-to)1280-1295
Number of pages16
JournalMolecular Oncology
Volume13
Issue number5
DOIs
Publication statusPublished - 2019 May 1

Fingerprint

HSC70 Heat-Shock Proteins
Ovarian Neoplasms
Snails
Down-Regulation
Neoplasm Metastasis
Ubiquitin
Neoplasms
Ubiquitin-Protein Ligases
Ligases
Proteins
Yeasts

Keywords

  • cancer metastasis
  • CHIP
  • E3 ubiquitin ligase
  • EMT
  • ovarian cancer
  • snail

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Oncology
  • Cancer Research

Cite this

Downregulation of CHIP promotes ovarian cancer metastasis by inducing Snail-mediated epithelial–mesenchymal transition. / Park, Sun Mi; Park, Seung Ho; Ryu, Ki Jun; Kim, In Kyu; Han, Hyeontak; Kim, Hyo Jin; Kim, Seon Hee; Hong, Keun Seok; Kim, Hyemin; Kim, Minju; Cho, Bok Im; Heo, Jeong Doo; Kim, Na Hyun; Hwang, Eun Mi; Park, Jae-Yong; Yook, Jong In; Cho, Hee Jun; Hwangbo, Cheol; Kim, Kwang Dong; Song, Hoseok; Yoo, Jiyun.

In: Molecular Oncology, Vol. 13, No. 5, 01.05.2019, p. 1280-1295.

Research output: Contribution to journalArticle

Park, SM, Park, SH, Ryu, KJ, Kim, IK, Han, H, Kim, HJ, Kim, SH, Hong, KS, Kim, H, Kim, M, Cho, BI, Heo, JD, Kim, NH, Hwang, EM, Park, J-Y, Yook, JI, Cho, HJ, Hwangbo, C, Kim, KD, Song, H & Yoo, J 2019, 'Downregulation of CHIP promotes ovarian cancer metastasis by inducing Snail-mediated epithelial–mesenchymal transition', Molecular Oncology, vol. 13, no. 5, pp. 1280-1295. https://doi.org/10.1002/1878-0261.12485
Park, Sun Mi ; Park, Seung Ho ; Ryu, Ki Jun ; Kim, In Kyu ; Han, Hyeontak ; Kim, Hyo Jin ; Kim, Seon Hee ; Hong, Keun Seok ; Kim, Hyemin ; Kim, Minju ; Cho, Bok Im ; Heo, Jeong Doo ; Kim, Na Hyun ; Hwang, Eun Mi ; Park, Jae-Yong ; Yook, Jong In ; Cho, Hee Jun ; Hwangbo, Cheol ; Kim, Kwang Dong ; Song, Hoseok ; Yoo, Jiyun. / Downregulation of CHIP promotes ovarian cancer metastasis by inducing Snail-mediated epithelial–mesenchymal transition. In: Molecular Oncology. 2019 ; Vol. 13, No. 5. pp. 1280-1295.
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AU - Cho, Hee Jun

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AU - Kim, Kwang Dong

AU - Song, Hoseok

AU - Yoo, Jiyun

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