Drastic anthocyanin increase in response to PAP1 overexpression in fls1 knockout mutant confers enhanced osmotic stress tolerance in Arabidopsis thaliana

Won Je Lee, Chan Young Jeong, Jaeyoung Kwon, Vu van Kien, Dongho Lee, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Key message: pap1-D/fls1kodouble mutant plants that produce substantial amounts of anthocyanin show tolerance to abiotic stress.Abstract: Anthocyanins are flavonoids that are abundant in various plants and have beneficial effects on both plants and humans. Many genes in flavonoid biosynthetic pathways have been identified, including those in the MYB-bHLH-WD40 (MBW) complex. The MYB gene Production of Anthocyanin Pigment 1 (PAP1) plays a particularly important role in anthocyanin accumulation. PAP1 expression in many plant systems strongly increases anthocyanin levels, resulting in a dark purple color in many plant organs. In this study, we generated double mutant plants that harbor fls1ko in the pap1-D background (i.e., pap1-D/fls1ko plants), to examine whether anthocyanins can be further enhanced by blocking flavonol biosynthesis under PAP1 overexpression. We also wanted to examine whether the increased anthocyanin levels contribute to defense against osmotic stresses. The pap1-D/fls1ko mutants accumulated higher anthocyanin levels than pap1-D plants in both control and sucrose-treated conditions. However, flavonoid biosynthesis genes were slightly down-regulated in the pap1-D/fls1ko seedlings as compared to their expression in pap1-D seedlings. We also report the performance of pap1-D/fls1ko seedlings in response to plant osmotic stresses.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalPlant Cell Reports
DOIs
Publication statusAccepted/In press - 2016 Aug 25

Fingerprint

knockout mutants
osmotic stress
stress tolerance
anthocyanins
Arabidopsis thaliana
pigments
flavonoids
mutants
seedlings
biosynthesis
genes
plant organs
flavonols
abiotic stress
biochemical pathways
sucrose

Keywords

  • Anthocyanins
  • Arabidopsis
  • Flavonoids
  • Osmotic stress
  • PAP1

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Drastic anthocyanin increase in response to PAP1 overexpression in fls1 knockout mutant confers enhanced osmotic stress tolerance in Arabidopsis thaliana. / Lee, Won Je; Jeong, Chan Young; Kwon, Jaeyoung; van Kien, Vu; Lee, Dongho; Hong, Suk Whan; Lee, Hojoung.

In: Plant Cell Reports, 25.08.2016, p. 1-11.

Research output: Contribution to journalArticle

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