@article{5d5368ed2e3c4306927c065d427bf00f,
title = "DRG2 deficient mice exhibit impaired motor behaviors with reduced striatal dopamine release",
abstract = "Developmentally regulated GTP-binding protein 2 (DRG2) was first identified in the central nervous system of mice. However, the physiological function of DRG2 in the brain remains largely unknown. Here, we demonstrated that knocking out DRG2 impairs the function of dopamine neurons in mice. DRG2 was strongly expressed in the neurons of the dopaminergic system such as those in the striatum (Str), ventral tegmental area (VTA), and substantia nigra (SN), and on neuronal cell bodies in high-density regions such as the hippocampus (HIP), cerebellum, and cerebral cortex in the mouse brain. DRG2 knockout (KO) mice displayed defects in motor function in motor coordination and rotarod tests and increased anxiety. However, unexpectedly, DRG2 depletion did not affect the dopamine (DA) neuron population in the SN, Str, or VTA region or dopamine synthesis in the Str region. We further demonstrated that dopamine release was significantly diminished in the Str region of DRG2 KO mice and that treatment of DRG2 KO mice with l-3,4-dihydroxyphenylalanine (L-DOPA), a dopamine precursor, rescued the behavioral motor deficiency in DRG2 KO mice as observed with the rotarod test. This is the first report to identify DRG2 as a key regulator of dopamine release from dopamine neurons in the mouse brain.",
keywords = "Developmentally regulated GTP-binding protein 2 (DRG2), Dopamine release, Dopaminergic neurons, Motor coordination, Motor deficiency, Striatum",
author = "Lim, {Hye Ryeong} and Vo, {Mai Tram} and Kim, {Dong Jun} and Lee, {Unn Hwa} and Yoon, {Jong Hyuk} and Kim, {Hyung Jun} and Jeongah Kim and Kim, {Sang Ryong} and Lee, {Jun Yeon} and Yang, {Chae Ha} and Kim, {Hee Young} and Choi, {June Seek} and Kijeong Kim and Esther Yang and Hyun Kim and Seongsoo Lee and Lee, {Byung Ju} and Kyungjin Kim and Park, {Jeong Woo} and Ha, {Chang Man}",
note = "Funding Information: Funding: This study was supported by the KBRI basic research program through the Korea Brain Research Institute funded by the Ministry of Science and ICT (No. 19-BR-03-03, 19-BR-02-03, 19-BR-04-02); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, South Korea (grant number: H I14C1135), and the Korean Government (MSIP) through the National Research Foundation of Korea (NRF-2015R1D 1A 1A01060561) to CMH, (NRF-2014R1A6A1030318) to JWP. Funding Information: This study was supported by the KBRI basic research program through the Korea Brain Research Institute funded by the Ministry of Science and ICT (No. 19-BR-03-03, 19-BR-02-03, 19-BR-04-02); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, South Korea (grant number: H I14C1135), and the Korean Government (MSIP) through the National Research Foundation of Korea (NRF-2015R1D 1A 1A01060561) to CMH, (NRF-2014R1A6A1030318) to JWP. All image equipment were supported by the Brain Research Core Facilities in KBRI and we thank Youngshik Choe?s lab and Hyun Jin Jung for their help with the HPLC experiment. Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = jan,
day = "1",
doi = "10.3390/ijms21010060",
language = "English",
volume = "21",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "1",
}