Droplet-based magnetically activated cell separation

Y. H. Kim, Chang Seop Hong, B. Kim, S. Yun, Y. R. Kang, K. K. Paek, J. W. Lee, Sang Ho Lee, Byeong Kwon Ju

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

In this study, we developed a method that target cells in suspension can be separated by combining magnetic force and gravitation force. Since the newly developed method involves a separating process of a droplet containing non-target cells in suspension by applying magnetic force to separate target cells, we called it droplet-based magnetic activated cell sorting (dMACS). To demonstrate the efficiency of the dMACS system, Ter119 (+) cells from mouse bone marrow cells were separated by both conventional MACS and our dMACS systems. Effects of three parameters on separation efficiency were examined in the dMACS system. As a result, both volume of droplet of cell suspension, and magnetic force did not affect the efficiency of cell separation markedly. However, the time for cell settlement in the droplet showed a critical role in the efficiency of cell separation according to increasing time. Therefore, we tried to verify that the saturation time affected increase of its efficiency and that flow rate injected to get rid of the negative cell resulted in the decrease of its efficiency. Using this dMACS system, we were able to pinpoint that the flow rate of cell suspension injected into a magnetic platform results in disturbance in the droplet, leading to turbulence in the cell suspension.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages2575-2578
Number of pages4
Volume26 IV
Publication statusPublished - 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: 2004 Sep 12004 Sep 5

Other

OtherConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004
CountryUnited States
CitySan Francisco, CA
Period04/9/104/9/5

Fingerprint

Sorting
Suspensions
Cells
Flow rate
Gravitation
Bone
Turbulence

Keywords

  • Cell separation
  • Droplet
  • MACS
  • Magnet

ASJC Scopus subject areas

  • Bioengineering

Cite this

Kim, Y. H., Hong, C. S., Kim, B., Yun, S., Kang, Y. R., Paek, K. K., ... Ju, B. K. (2004). Droplet-based magnetically activated cell separation. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 26 IV, pp. 2575-2578)

Droplet-based magnetically activated cell separation. / Kim, Y. H.; Hong, Chang Seop; Kim, B.; Yun, S.; Kang, Y. R.; Paek, K. K.; Lee, J. W.; Lee, Sang Ho; Ju, Byeong Kwon.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 IV 2004. p. 2575-2578.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, YH, Hong, CS, Kim, B, Yun, S, Kang, YR, Paek, KK, Lee, JW, Lee, SH & Ju, BK 2004, Droplet-based magnetically activated cell separation. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 26 IV, pp. 2575-2578, Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004, San Francisco, CA, United States, 04/9/1.
Kim YH, Hong CS, Kim B, Yun S, Kang YR, Paek KK et al. Droplet-based magnetically activated cell separation. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 IV. 2004. p. 2575-2578
Kim, Y. H. ; Hong, Chang Seop ; Kim, B. ; Yun, S. ; Kang, Y. R. ; Paek, K. K. ; Lee, J. W. ; Lee, Sang Ho ; Ju, Byeong Kwon. / Droplet-based magnetically activated cell separation. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 IV 2004. pp. 2575-2578
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