Droplet squeezing for highly effective gene delivery into human t lymphocytes

Byeongju Joo, Hasung Lee, Seung Gyu Yun, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel droplet-based intracellular delivery method for highly effective internalization of external macromolecules into human T lymphocytes. First, we generated droplets that encapsulate single cells mixed with biomolecules, using a flow-focusing droplet generator. The droplets containing cells were then passed through a series of narrow constrictions, creating transient nanopores in the cell membrane. Consequently, external cargos in droplets can be transported directly into the cell cytosol throughout the nanopores' lifespan, achieving effective intracellular delivery. This process was able to achieve highly efficient and high-throughput delivery of different nanomaterials into human T lymphocytes, while maintaining high cell viability.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages100-101
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 2020 Oct 42020 Oct 9

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period20/10/420/10/9

Keywords

  • Droplet Microfluidics
  • Droplet Squeezing
  • Gene Delivery
  • Intracellular Delivery

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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