DROPLET SQUEEZING FOR HUMAN PRIMARY T CELL TRANSFECTION

Byeongju Joo, Jeongsoo Hur, Gi Beom Kim, Seung Gyu Yun, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel microfluidic intracellular delivery approach termed droplet squeezing, leveraging droplet microfluidics with mechanical cell permeabilization. Briefly, cells and target nanomaterials are co-encapsulated within droplets that pass through a series of constrictions for cell mechanoporation, enabling a highly efficient internalization of external nanomaterials into cells. The use of droplet reduces the external biomolecule consumption drastically and provides near-zero clogging operations. Using the presented droplet squeezing method, mRNAs, plasmid DNAs, and CRISPR-Cas9 ribonucleoproteins were delivered into various cell types, and highly effective primary T cell genomic editing was also demonstrated, showing high potential for T cell-based therapeutic applications [1].

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages117-118
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Keywords

  • Droplet Microfluidics
  • Droplet Squeezing
  • Gene Delivery
  • Intracellular Delivery

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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