TY - GEN
T1 - DROPLET SQUEEZING FOR HUMAN PRIMARY T CELL TRANSFECTION
AU - Joo, Byeongju
AU - Hur, Jeongsoo
AU - Kim, Gi Beom
AU - Yun, Seung Gyu
AU - Chung, Aram
N1 - Funding Information:
This study was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (NRF-2021R1A2C2006224).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We present a novel microfluidic intracellular delivery approach termed droplet squeezing, leveraging droplet microfluidics with mechanical cell permeabilization. Briefly, cells and target nanomaterials are co-encapsulated within droplets that pass through a series of constrictions for cell mechanoporation, enabling a highly efficient internalization of external nanomaterials into cells. The use of droplet reduces the external biomolecule consumption drastically and provides near-zero clogging operations. Using the presented droplet squeezing method, mRNAs, plasmid DNAs, and CRISPR-Cas9 ribonucleoproteins were delivered into various cell types, and highly effective primary T cell genomic editing was also demonstrated, showing high potential for T cell-based therapeutic applications [1].
AB - We present a novel microfluidic intracellular delivery approach termed droplet squeezing, leveraging droplet microfluidics with mechanical cell permeabilization. Briefly, cells and target nanomaterials are co-encapsulated within droplets that pass through a series of constrictions for cell mechanoporation, enabling a highly efficient internalization of external nanomaterials into cells. The use of droplet reduces the external biomolecule consumption drastically and provides near-zero clogging operations. Using the presented droplet squeezing method, mRNAs, plasmid DNAs, and CRISPR-Cas9 ribonucleoproteins were delivered into various cell types, and highly effective primary T cell genomic editing was also demonstrated, showing high potential for T cell-based therapeutic applications [1].
KW - Droplet Microfluidics
KW - Droplet Squeezing
KW - Gene Delivery
KW - Intracellular Delivery
UR - http://www.scopus.com/inward/record.url?scp=85136927478&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136927478
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 117
EP - 118
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -