Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis

Cheong Hoon Kwon, Ian Wheeldon, Nezamoddin N. Kachouie, Seung Hwan Lee, Hojae Bae, Shilpa Sant, Junji Fukuda, Jeong Won Kang, Ali Khademhosseini

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Traditional high-throughput screening (HTS) is carried out in centralized facilities that require extensive robotic liquid and plate handling equipment. This model of HTS is restrictive as such facilities are not accessible to many researchers. We have designed a simple microarray platform for cell-based screening that can be carried out at the benchtop. The device creates a microarray of 2100 individual cell-based assays in a standard microscope slide format. A microarray of chemical-laden hydrogels addresses a matching array of cell-laden microwells thus creating a microarray of sealed microscale cell cultures each with unique conditions. We demonstrate the utility of the device by screening the extent of apoptosis and necrosis in MCF-7 breast cancer cells in response to exposure to a small library of chemical compounds. From a set of screens we produced a rank order of chemicals that preferentially induce apoptosis over necrosis in MCF-7 cells. Treatment with doxorubicin induced high levels of apoptosis in comparison with staurosporine, ethanol, and hydrogen peroxide, whereas treatment with 100 μM ethanol induced minimal apoptosis with high levels of necrosis. We anticipate broad application of the device for various research and discovery applications as it is easy to use, scalable, and can be fabricated and operated with minimal peripheral equipment.

Original languageEnglish
Pages (from-to)4118-4125
Number of pages8
JournalAnalytical Chemistry
Volume83
Issue number11
DOIs
Publication statusPublished - 2011 Jun 1

Fingerprint

Microarrays
Screening
Apoptosis
Pharmaceutical Preparations
Ethanol
Throughput
Hydrogels
Chemical compounds
Staurosporine
Cell culture
Doxorubicin
Hydrogen Peroxide
Assays
Robotics
Microscopes
Cells
Liquids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kwon, C. H., Wheeldon, I., Kachouie, N. N., Lee, S. H., Bae, H., Sant, S., ... Khademhosseini, A. (2011). Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis. Analytical Chemistry, 83(11), 4118-4125. https://doi.org/10.1021/ac200267t

Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis. / Kwon, Cheong Hoon; Wheeldon, Ian; Kachouie, Nezamoddin N.; Lee, Seung Hwan; Bae, Hojae; Sant, Shilpa; Fukuda, Junji; Kang, Jeong Won; Khademhosseini, Ali.

In: Analytical Chemistry, Vol. 83, No. 11, 01.06.2011, p. 4118-4125.

Research output: Contribution to journalArticle

Kwon, CH, Wheeldon, I, Kachouie, NN, Lee, SH, Bae, H, Sant, S, Fukuda, J, Kang, JW & Khademhosseini, A 2011, 'Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis', Analytical Chemistry, vol. 83, no. 11, pp. 4118-4125. https://doi.org/10.1021/ac200267t
Kwon CH, Wheeldon I, Kachouie NN, Lee SH, Bae H, Sant S et al. Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis. Analytical Chemistry. 2011 Jun 1;83(11):4118-4125. https://doi.org/10.1021/ac200267t
Kwon, Cheong Hoon ; Wheeldon, Ian ; Kachouie, Nezamoddin N. ; Lee, Seung Hwan ; Bae, Hojae ; Sant, Shilpa ; Fukuda, Junji ; Kang, Jeong Won ; Khademhosseini, Ali. / Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis. In: Analytical Chemistry. 2011 ; Vol. 83, No. 11. pp. 4118-4125.
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