TY - JOUR
T1 - Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy
AU - Kim, Dojin
AU - Jeong, Keunsoo
AU - Kwon, Ji Eon
AU - Park, Hyeonjong
AU - Lee, Seokyung
AU - Kim, Sehoon
AU - Park, Soo Young
N1 - Funding Information:
This work was supported by the Creative Research Initiative Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP) (Grant No. 2009-0081571[RIAM0417-20170011]), and the Basic Science Research Program through the NRF funded by the MSIP (Grant No. 2017R1E1A1A01075372). S.K. thanks Korea Research Institute of Standards and Science (KRISS-2018-GP2018-0018) for KIST intramural program. We thank Jinhoe Hur and Hongchan Joung (Optical Biomed Imaging Center, Ulsan National Institute of Science and Technology (UNIST), Korea) for technical support and discussions on PALM imaging and analysis.
Publisher Copyright:
© 2019, The Author(s).
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.
AB - Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.
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U2 - 10.1038/s41467-019-10986-4
DO - 10.1038/s41467-019-10986-4
M3 - Article
C2 - 31300649
AN - SCOPUS:85068992338
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 3089
ER -