Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy

Dojin Kim, Keunsoo Jeong, Ji Eon Kwon, Hyeonjong Park, Seokyung Lee, Sehoon Kim, Soo Young Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.

Original languageEnglish
Article number3089
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Nanoparticles
Microscopy
Microscopic examination
Color
Energy Transfer
microscopy
color
nanoparticles
Proton transfer
Protons
Polymers
Fluorescence
Excited states
Energy transfer
dyes
energy transfer
Ultraviolet Rays
Fluorescent Dyes
Imaging techniques
fluorescence

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy. / Kim, Dojin; Jeong, Keunsoo; Kwon, Ji Eon; Park, Hyeonjong; Lee, Seokyung; Kim, Sehoon; Park, Soo Young.

In: Nature communications, Vol. 10, No. 1, 3089, 01.12.2019.

Research output: Contribution to journalArticle

Kim, Dojin ; Jeong, Keunsoo ; Kwon, Ji Eon ; Park, Hyeonjong ; Lee, Seokyung ; Kim, Sehoon ; Park, Soo Young. / Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy. In: Nature communications. 2019 ; Vol. 10, No. 1.
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