Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes

Xiaohu Dong, Ji Hee Han, Cheol Ho Heo, Hwan Myung Kim, Zhihong Liu, Bong-Rae Cho

Research output: Contribution to journal › Article

42 Citations (Scopus)

Abstract

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+]i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+]m), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+]i /[Ca2+]m distributions in live tissues at a depth of 100-200 μm.

Original languageEnglish
Pages (from-to)8110-8113
Number of pages4
JournalAnalytical Chemistry
Volume84
Issue number19
DOIs
Publication statusPublished - 2012 Oct 2

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Fluorescent Dyes
Magnesium
Photons
Ions
Calcium
Color
Imaging techniques
Tissue
Microscopic examination
Membranes

ASJC Scopus subject areas

  • Analytical Chemistry

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Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes. / Dong, Xiaohu; Han, Ji Hee; Heo, Cheol Ho; Kim, Hwan Myung; Liu, Zhihong; Cho, Bong-Rae.

In: Analytical Chemistry, Vol. 84, No. 19, 02.10.2012, p. 8110-8113.

Research output: Contribution to journal › Article

Dong, X, Han, JH, Heo, CH, Kim, HM, Liu, Z & Cho, B-R 2012, 'Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes', Analytical Chemistry, vol. 84, no. 19, pp. 8110-8113. https://doi.org/10.1021/ac302210v
Dong X, Han JH, Heo CH, Kim HM, Liu Z, Cho B-R. Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes. Analytical Chemistry. 2012 Oct 2;84(19):8110-8113. https://doi.org/10.1021/ac302210v
Dong, Xiaohu ; Han, Ji Hee ; Heo, Cheol Ho ; Kim, Hwan Myung ; Liu, Zhihong ; Cho, Bong-Rae. / Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes. In: Analytical Chemistry. 2012 ; Vol. 84, No. 19. pp. 8110-8113.
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