Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes

Xiaohu Dong, Ji Hee Han, Cheol Ho Heo, Hwan Myung Kim, Zhihong Liu, Bong Rae Cho

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+]i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+]m), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+]i /[Ca2+]m distributions in live tissues at a depth of 100-200 μm.

Original languageEnglish
Pages (from-to)8110-8113
Number of pages4
JournalAnalytical chemistry
Volume84
Issue number19
DOIs
Publication statusPublished - 2012 Oct 2

ASJC Scopus subject areas

  • Analytical Chemistry

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