Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes

Xiaohu Dong; Ji Hee Han; Cheol Ho Heo; Hwan Myung Kim; Zhihong Liu; Bong Rae Cho

doi:10.1021/ac302210v

Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes

Xiaohu Dong, Ji Hee Han, Cheol Ho Heo, Hwan Myung Kim, Zhihong Liu, Bong Rae Cho

Honorary professors

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg²⁺ ([Mg²⁺]_i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca²⁺ ([Ca²⁺]_m), FMg2 allows dual-color imaging of Mg²⁺/Ca²⁺ activities in live cells and [Mg²⁺]_i /[Ca²⁺]_m distributions in live tissues at a depth of 100-200 μm.

Original language	English
Pages (from-to)	8110-8113
Number of pages	4
Journal	Analytical chemistry
Volume	84
Issue number	19
DOIs	https://doi.org/10.1021/ac302210v
Publication status	Published - 2012 Oct 2

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac302210v

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abstract = "We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+]i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+]m), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+]i /[Ca2+]m distributions in live tissues at a depth of 100-200 μm.",

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AU - Dong, Xiaohu

AU - Han, Ji Hee

AU - Heo, Cheol Ho

AU - Kim, Hwan Myung

AU - Liu, Zhihong

AU - Cho, Bong Rae

PY - 2012/10/2

Y1 - 2012/10/2

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AB - We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+]i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+]m), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+]i /[Ca2+]m distributions in live tissues at a depth of 100-200 μm.

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