Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes

Xiaohu Dong; Ji Hee Han; Cheol Ho Heo; Hwan Myung Kim; Zhihong Liu; Bong Rae Cho

doi:10.1021/ac302210v

Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes

Xiaohu Dong, Ji Hee Han, Cheol Ho Heo, Hwan Myung Kim, Zhihong Liu, Bong Rae Cho

Honorary professors

Research output: Contribution to journal › Article

43 Citations (Scopus)

Abstract

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg²⁺ ([Mg²⁺]_i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca²⁺ ([Ca²⁺]_m), FMg2 allows dual-color imaging of Mg²⁺/Ca²⁺ activities in live cells and [Mg²⁺]_i /[Ca²⁺]_m distributions in live tissues at a depth of 100-200 μm.

Original language	English
Pages (from-to)	8110-8113
Number of pages	4
Journal	Analytical chemistry
Volume	84
Issue number	19
DOIs	https://doi.org/10.1021/ac302210v
Publication status	Published - 2012 Oct 2

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac302210v

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Dong, X., Han, J. H., Heo, C. H., Kim, H. M., Liu, Z., & Cho, B. R. (2012). Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes. Analytical chemistry, 84(19), 8110-8113. https://doi.org/10.1021/ac302210v

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AU - Han, Ji Hee

AU - Heo, Cheol Ho

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AU - Liu, Zhihong

AU - Cho, Bong Rae

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