Dual-fluorophore Raspberry-like Nanohybrids for Ratiometric pH Sensing

Isaac Acquah, Jinkyu Roh, Dong June Ahn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report on the development of raspberry-like silica structures formed by the adsorption of 8-hydroxypyrene-1,3,6-trisulfonate (HPTS)@silica nanoparticles (NPs) on rhodamine B isothiocyanate (RBTIC)@silica NPs for ratiometric fluorescence-based pH sensing. To overcome the well-known problem of dye leaching which occurs during encapsulation of anionic HPTS dye in silica NPs, we utilized a polyelectrolyte-assisted incorporation of the anionic HPTS. The morphological and optical characterization of the as-synthesized dye-doped NPs and the resulting nanohybrids were carried out. The pH-sensitive dye, HPTS, incorporated in the HPTS-doped silica NPs provided a pH-dependent fluorescence response while the RBITC-doped silica provided the reference signal for ratiometric sensing. We evaluated the effectiveness of the nanohybrids for pH sensing; the ratio of the fluorescence emission intensity at 510 nm and 583 nm at excitation wavelengths of 454 nm and 555 nm, respectively. The results showed a dynamic response in the acidic pH range. With this approach, nanohybrids containing different dyes or receptors could be developed for multifunctioning and multiplexing applications.

Original languageEnglish
Pages (from-to)1724-1729
Number of pages6
JournalChemistry - An Asian Journal
Volume12
Issue number14
DOIs
Publication statusPublished - 2017 Jul 18

Fingerprint

Fluorophores
Silicon Dioxide
Coloring Agents
Nanoparticles
Fluorescence
Polyelectrolytes
Multiplexing
Encapsulation
Leaching
Dynamic response
Adsorption
Wavelength
1-hydroxypyrene

Keywords

  • dual-fluorophore
  • polyelectrolyte
  • raspberry structure
  • ratiometric pH sensors
  • silica nanohybrids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Dual-fluorophore Raspberry-like Nanohybrids for Ratiometric pH Sensing. / Acquah, Isaac; Roh, Jinkyu; Ahn, Dong June.

In: Chemistry - An Asian Journal, Vol. 12, No. 14, 18.07.2017, p. 1724-1729.

Research output: Contribution to journalArticle

Acquah, Isaac ; Roh, Jinkyu ; Ahn, Dong June. / Dual-fluorophore Raspberry-like Nanohybrids for Ratiometric pH Sensing. In: Chemistry - An Asian Journal. 2017 ; Vol. 12, No. 14. pp. 1724-1729.
@article{d29f13ca9d3a47a789a46c15d18cb179,
title = "Dual-fluorophore Raspberry-like Nanohybrids for Ratiometric pH Sensing",
abstract = "We report on the development of raspberry-like silica structures formed by the adsorption of 8-hydroxypyrene-1,3,6-trisulfonate (HPTS)@silica nanoparticles (NPs) on rhodamine B isothiocyanate (RBTIC)@silica NPs for ratiometric fluorescence-based pH sensing. To overcome the well-known problem of dye leaching which occurs during encapsulation of anionic HPTS dye in silica NPs, we utilized a polyelectrolyte-assisted incorporation of the anionic HPTS. The morphological and optical characterization of the as-synthesized dye-doped NPs and the resulting nanohybrids were carried out. The pH-sensitive dye, HPTS, incorporated in the HPTS-doped silica NPs provided a pH-dependent fluorescence response while the RBITC-doped silica provided the reference signal for ratiometric sensing. We evaluated the effectiveness of the nanohybrids for pH sensing; the ratio of the fluorescence emission intensity at 510 nm and 583 nm at excitation wavelengths of 454 nm and 555 nm, respectively. The results showed a dynamic response in the acidic pH range. With this approach, nanohybrids containing different dyes or receptors could be developed for multifunctioning and multiplexing applications.",
keywords = "dual-fluorophore, polyelectrolyte, raspberry structure, ratiometric pH sensors, silica nanohybrids",
author = "Isaac Acquah and Jinkyu Roh and Ahn, {Dong June}",
year = "2017",
month = "7",
day = "18",
doi = "10.1002/asia.201700616",
language = "English",
volume = "12",
pages = "1724--1729",
journal = "Chemistry - An Asian Journal",
issn = "1861-4728",
publisher = "John Wiley and Sons Ltd",
number = "14",

}

TY - JOUR

T1 - Dual-fluorophore Raspberry-like Nanohybrids for Ratiometric pH Sensing

AU - Acquah, Isaac

AU - Roh, Jinkyu

AU - Ahn, Dong June

PY - 2017/7/18

Y1 - 2017/7/18

N2 - We report on the development of raspberry-like silica structures formed by the adsorption of 8-hydroxypyrene-1,3,6-trisulfonate (HPTS)@silica nanoparticles (NPs) on rhodamine B isothiocyanate (RBTIC)@silica NPs for ratiometric fluorescence-based pH sensing. To overcome the well-known problem of dye leaching which occurs during encapsulation of anionic HPTS dye in silica NPs, we utilized a polyelectrolyte-assisted incorporation of the anionic HPTS. The morphological and optical characterization of the as-synthesized dye-doped NPs and the resulting nanohybrids were carried out. The pH-sensitive dye, HPTS, incorporated in the HPTS-doped silica NPs provided a pH-dependent fluorescence response while the RBITC-doped silica provided the reference signal for ratiometric sensing. We evaluated the effectiveness of the nanohybrids for pH sensing; the ratio of the fluorescence emission intensity at 510 nm and 583 nm at excitation wavelengths of 454 nm and 555 nm, respectively. The results showed a dynamic response in the acidic pH range. With this approach, nanohybrids containing different dyes or receptors could be developed for multifunctioning and multiplexing applications.

AB - We report on the development of raspberry-like silica structures formed by the adsorption of 8-hydroxypyrene-1,3,6-trisulfonate (HPTS)@silica nanoparticles (NPs) on rhodamine B isothiocyanate (RBTIC)@silica NPs for ratiometric fluorescence-based pH sensing. To overcome the well-known problem of dye leaching which occurs during encapsulation of anionic HPTS dye in silica NPs, we utilized a polyelectrolyte-assisted incorporation of the anionic HPTS. The morphological and optical characterization of the as-synthesized dye-doped NPs and the resulting nanohybrids were carried out. The pH-sensitive dye, HPTS, incorporated in the HPTS-doped silica NPs provided a pH-dependent fluorescence response while the RBITC-doped silica provided the reference signal for ratiometric sensing. We evaluated the effectiveness of the nanohybrids for pH sensing; the ratio of the fluorescence emission intensity at 510 nm and 583 nm at excitation wavelengths of 454 nm and 555 nm, respectively. The results showed a dynamic response in the acidic pH range. With this approach, nanohybrids containing different dyes or receptors could be developed for multifunctioning and multiplexing applications.

KW - dual-fluorophore

KW - polyelectrolyte

KW - raspberry structure

KW - ratiometric pH sensors

KW - silica nanohybrids

UR - http://www.scopus.com/inward/record.url?scp=85021157424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021157424&partnerID=8YFLogxK

U2 - 10.1002/asia.201700616

DO - 10.1002/asia.201700616

M3 - Article

VL - 12

SP - 1724

EP - 1729

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

SN - 1861-4728

IS - 14

ER -