Dual inhibitions of lemon balm (Melissa officinalis) ethanolic extract on melanogenesis in B16-F1 murine melanocytes: Inhibition of tyrosinase activity and its gene expression

Hee jin Jun, Miran Roh, Hae Won Kim, Soung Jin Houng, Boram Cho, Eun Joo Yun, Md A. Hossain, Hojoung Lee, Kyoung Heon Kim, Sung-Joon Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effects of wild type and UV-irradiated lemon balm (Melissa officinalis) ethanolic extracts (MOE and UMOE) on melanogenesis in vitro were examined. UMOE showed potent antioxidant activity and significantly inhibited the mushroom and melanocyte tyrosinase activity, and lowered cellular melanin content by 49% at 200 μg/mL in B16-F1 melanocytes. The key gene and protein expression of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 were reduced (-73% for TRP-1 protein at 200 μg/mL UMOE, p<0.05). MOE showed similar results to a slightly lesser degree. We found that myo-inositol, a major compound in lemon balm extracts, significantly reduced cellular melanin synthesis and its effect was greater than arbutin at 1 mM. These suggest that both MOE and UMOE have anti-melanogenic role by both direct inhibition of tyrosinase and down-regulation of gene expressions in melanogenesis. UV-irradiation slightly improved the anti-melanogenic activities. UMOE may be useful as natural anti-melanogenic biomaterials for functional foods and cosmetics.

Original languageEnglish
Pages (from-to)1051-1059
Number of pages9
JournalFood Science and Biotechnology
Volume20
Issue number4
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Melissa
lemon balm
Melissa officinalis
melanogenesis
melanocytes
Monophenol Monooxygenase
Melanocytes
Melanins
Gene Expression
gene expression
mice
extracts
melanin
Arbutin
Functional Food
proteins
Agaricales
Gene Expression Regulation
Biocompatible Materials
Inositol

Keywords

  • hypopigmentation
  • lemon balm (Melissa officinalis)
  • tyrosinase
  • UV-irradiation

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Dual inhibitions of lemon balm (Melissa officinalis) ethanolic extract on melanogenesis in B16-F1 murine melanocytes : Inhibition of tyrosinase activity and its gene expression. / Jun, Hee jin; Roh, Miran; Kim, Hae Won; Houng, Soung Jin; Cho, Boram; Yun, Eun Joo; Hossain, Md A.; Lee, Hojoung; Kim, Kyoung Heon; Lee, Sung-Joon.

In: Food Science and Biotechnology, Vol. 20, No. 4, 01.08.2011, p. 1051-1059.

Research output: Contribution to journalArticle

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