Dual-Modal Imaging-Guided Precise Tracking of Bioorthogonally Labeled Mesenchymal Stem Cells in Mouse Brain Stroke

Seungho Lim, Hong Yeol Yoon, Hee Jeong Jang, Sukyung Song, Woojun Kim, Jooho Park, Kyung Eun Lee, Sangmin Jeon, Sangmin Lee, Dong Kwon Lim, Byung Soo Kim, Dong Eog Kim, Kwangmeyung Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Noninvasive and precise stem cell tracking after transplantation in living subject is very important to monitor both stem cell destinations and their in vivo fate, which is closely related to their therapeutic efficacy. Herein, we developed bicyclo[6.1.0]nonyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-NPs) as a delivery system of dual-modal stem cell imaging probes. Near-infrared fluorescent (NIRF) dye Cy5.5 was chemically conjugated to the BCN-NPs, and then oleic acid-coated superparamagnetic iron oxide nanoparticles (OA-Fe3O4 NPs) were encapsulated into BCN-NPs, resulting in Cy5.5-labeled and OA-Fe3O4 NP-encapsulated BCN-NPs (BCN-dual-NPs). For bioorthogonal labeling of human adipose-derived mesenchymal stem cells (hMSCs), first, hMSCs were treated with tetra-acetylated N-azidoacetyl-d-mannosamine (Ac4ManNAz) for generating azide (-N3) groups onto their surface via metabolic glycoengineering. Second, azide groups on the cell surface were successfully chemically labeled with BCN-dual-NPs via bioorthogonal click chemistry in vitro. This bioorthogonal labeling of hMSCs could greatly increase the cell labeling efficiency, safety, and imaging sensitivity, compared to only nanoparticle-derived labeling technology. The dual-modal imaging-guided precise tracking of bioorthogonally labeled hMSCs was tested in the photothrombotic stroke mouse model via intraparenchymal injection. Finally, BCN-dual-NPs-labeled hMSCs could be effectively tracked by their migration from the implanted site to the brain stroke lesion using NIRF/T2-weighted magnetic resonance (MR) dual-modal imaging for 14 days. Our observation would provide a potential application of bioorthogonally labeled stem cell imaging in regenerative medicine by providing safety and high labeling efficiency in vitro and in vivo.

Original languageEnglish
Pages (from-to)10991-11007
Number of pages17
JournalACS Nano
Volume13
Issue number10
DOIs
Publication statusPublished - 2019 Oct 22

Keywords

  • bioorthogonal click chemistry
  • brain stroke
  • dual-modal imaging
  • imaging probe
  • metabolic engineering
  • stem cell tracking

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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  • Cite this

    Lim, S., Yoon, H. Y., Jang, H. J., Song, S., Kim, W., Park, J., Lee, K. E., Jeon, S., Lee, S., Lim, D. K., Kim, B. S., Kim, D. E., & Kim, K. (2019). Dual-Modal Imaging-Guided Precise Tracking of Bioorthogonally Labeled Mesenchymal Stem Cells in Mouse Brain Stroke. ACS Nano, 13(10), 10991-11007. https://doi.org/10.1021/acsnano.9b02173