Dual-modality in vivo imaging for MRI detection of tumors and NIRF-guided surgery using multi-component nanoparticles

Jaehong Key, Kwang Meyung Kim, Deepika Dhawan, Deborah W. Knapp, Ick Chan Kwon, Kuiwon Choi, James F. Leary

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) is one of the best imaging modalities for noninvasive cancer detection but MRI does not have enough sensitivity to delineate tumor margins during surgery. Moreover, since most surgical tools contain metal substances, image-guided surgery is hard to perform with a MR machine using magnets. Also, MR imaging is too slow for real-time guided-surgery. On the other hand, near infrared fluorescence (NIRF) imaging has recently received great interest for in vivo imaging due to its high signal-to-noise ratios and short image-acquisition times. NIRF imaging can be used to delineate tumor margins during surgery, but current NIRF imaging cannot provide the penetration depth to detect early-stage cancer inside body. Thus, we have developed dual-modality in vivo imaging for MRI detection of tumors and NIRF-guided surgery using multi-component nanoparticles. NIRF dye (cyanine 5.5, Cy5.5), conjugated glycol chitosan nanoparticles (HGC) exhibited excellent tumor targeting ability with NIRF imaging. Superparamagnetic iron oxide (SPIO) nanoparticles as a MR contrast agent were loaded into the nanoparticles, resulting in SPIO-HGC-Cy5.5 nanoparticles. SPIO-HGC-Cy5.5 nanoparticles were characterized and evaluated in mice by both NIRF and MR imaging. Our results indicate SPIO-HGC-Cy5.5 nanoparticles have the potential for dual-modality in vivo imaging with MRI detection of tumors and NIRF-guided surgery.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7908
DOIs
Publication statusPublished - 2011 Apr 1
Externally publishedYes
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII - San Francisco, CA, United States
Duration: 2011 Jan 242011 Jan 27

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
CountryUnited States
CitySan Francisco, CA
Period11/1/2411/1/27

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Key, J., Kim, K. M., Dhawan, D., Knapp, D. W., Kwon, I. C., Choi, K., & Leary, J. F. (2011). Dual-modality in vivo imaging for MRI detection of tumors and NIRF-guided surgery using multi-component nanoparticles. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7908). [790805] https://doi.org/10.1117/12.874241