Dual reporter genes enabling cell tracing with viable and reliable selection of various cell types

C. N. Hwang; S. Hong; S. S. Choi; K. S. Lee; S. S. Park; S. H. Lee

doi:10.1007/s10529-005-5715-9

Dual reporter genes enabling cell tracing with viable and reliable selection of various cell types

C. N. Hwang, S. Hong, S. S. Choi, K. S. Lee, S. S. Park, S. H. Lee

Honorary professors

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Dual reporter genes driven by either a ubiquitous cytomegalovirus (CMV) or a neuro-specific tubulin α1 promoter (Tα1) were constructed. The new genes, CMV (pCMV-GL) or Tα1 promoter-driven GFP-LacZ (pTα1-GL), robustly expressed the fused GFP-LacZ protein reporting constitutive expressions in various cell types including CHO cells, loach and chicken embryos, and neuro-specific expression in differentiating mouse embryonic stem cells, respectively. The dual reporter genes thus provide a versatile tool for the studies of gene expression, cell lineage within the embryo and possibly the fate of stem cells in transplantation experiment, thus facilitating different analyses depending on the experimental purposes.

Original language	English
Pages (from-to)	287-293
Number of pages	7
Journal	Biotechnology letters
Volume	28
Issue number	5
DOIs	https://doi.org/10.1007/s10529-005-5715-9
Publication status	Published - 2006 Mar

Keywords

Cytomegalovirus
Dual reporter gene
GFP-LacZ
Promoters
Stem cells tubulin

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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abstract = "Dual reporter genes driven by either a ubiquitous cytomegalovirus (CMV) or a neuro-specific tubulin α1 promoter (Tα1) were constructed. The new genes, CMV (pCMV-GL) or Tα1 promoter-driven GFP-LacZ (pTα1-GL), robustly expressed the fused GFP-LacZ protein reporting constitutive expressions in various cell types including CHO cells, loach and chicken embryos, and neuro-specific expression in differentiating mouse embryonic stem cells, respectively. The dual reporter genes thus provide a versatile tool for the studies of gene expression, cell lineage within the embryo and possibly the fate of stem cells in transplantation experiment, thus facilitating different analyses depending on the experimental purposes.",

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AU - Hwang, C. N.

AU - Hong, S.

AU - Choi, S. S.

AU - Lee, K. S.

AU - Park, S. S.

AU - Lee, S. H.

PY - 2006/3

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