Abstract
Dual reporter genes driven by either a ubiquitous cytomegalovirus (CMV) or a neuro-specific tubulin α1 promoter (Tα1) were constructed. The new genes, CMV (pCMV-GL) or Tα1 promoter-driven GFP-LacZ (pTα1-GL), robustly expressed the fused GFP-LacZ protein reporting constitutive expressions in various cell types including CHO cells, loach and chicken embryos, and neuro-specific expression in differentiating mouse embryonic stem cells, respectively. The dual reporter genes thus provide a versatile tool for the studies of gene expression, cell lineage within the embryo and possibly the fate of stem cells in transplantation experiment, thus facilitating different analyses depending on the experimental purposes.
| Original language | English |
|---|---|
| Pages (from-to) | 287-293 |
| Number of pages | 7 |
| Journal | Biotechnology Letters |
| Volume | 28 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 Mar 1 |
Fingerprint
Keywords
- Cytomegalovirus
- Dual reporter gene
- GFP-LacZ
- Promoters
- Stem cells tubulin
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
- Bioengineering
Cite this
Dual reporter genes enabling cell tracing with viable and reliable selection of various cell types. / Hwang, C. N.; Hong, S.; Choi, S. S.; Lee, K. S.; Park, Sung Soo; Lee, Sang Ho.
In: Biotechnology Letters, Vol. 28, No. 5, 01.03.2006, p. 287-293.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dual reporter genes enabling cell tracing with viable and reliable selection of various cell types
AU - Hwang, C. N.
AU - Hong, S.
AU - Choi, S. S.
AU - Lee, K. S.
AU - Park, Sung Soo
AU - Lee, Sang Ho
PY - 2006/3/1
Y1 - 2006/3/1
N2 - Dual reporter genes driven by either a ubiquitous cytomegalovirus (CMV) or a neuro-specific tubulin α1 promoter (Tα1) were constructed. The new genes, CMV (pCMV-GL) or Tα1 promoter-driven GFP-LacZ (pTα1-GL), robustly expressed the fused GFP-LacZ protein reporting constitutive expressions in various cell types including CHO cells, loach and chicken embryos, and neuro-specific expression in differentiating mouse embryonic stem cells, respectively. The dual reporter genes thus provide a versatile tool for the studies of gene expression, cell lineage within the embryo and possibly the fate of stem cells in transplantation experiment, thus facilitating different analyses depending on the experimental purposes.
AB - Dual reporter genes driven by either a ubiquitous cytomegalovirus (CMV) or a neuro-specific tubulin α1 promoter (Tα1) were constructed. The new genes, CMV (pCMV-GL) or Tα1 promoter-driven GFP-LacZ (pTα1-GL), robustly expressed the fused GFP-LacZ protein reporting constitutive expressions in various cell types including CHO cells, loach and chicken embryos, and neuro-specific expression in differentiating mouse embryonic stem cells, respectively. The dual reporter genes thus provide a versatile tool for the studies of gene expression, cell lineage within the embryo and possibly the fate of stem cells in transplantation experiment, thus facilitating different analyses depending on the experimental purposes.
KW - Cytomegalovirus
KW - Dual reporter gene
KW - GFP-LacZ
KW - Promoters
KW - Stem cells tubulin
UR - http://www.scopus.com/inward/record.url?scp=33645855601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645855601&partnerID=8YFLogxK
U2 - 10.1007/s10529-005-5715-9
DO - 10.1007/s10529-005-5715-9
M3 - Article
C2 - 16614914
AN - SCOPUS:33645855601
VL - 28
SP - 287
EP - 293
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 5
ER -