Dual reporter genes enabling cell tracing with viable and reliable selection of various cell types

C. N. Hwang, S. Hong, S. S. Choi, K. S. Lee, S. S. Park, S. H. Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dual reporter genes driven by either a ubiquitous cytomegalovirus (CMV) or a neuro-specific tubulin α1 promoter (Tα1) were constructed. The new genes, CMV (pCMV-GL) or Tα1 promoter-driven GFP-LacZ (pTα1-GL), robustly expressed the fused GFP-LacZ protein reporting constitutive expressions in various cell types including CHO cells, loach and chicken embryos, and neuro-specific expression in differentiating mouse embryonic stem cells, respectively. The dual reporter genes thus provide a versatile tool for the studies of gene expression, cell lineage within the embryo and possibly the fate of stem cells in transplantation experiment, thus facilitating different analyses depending on the experimental purposes.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalBiotechnology letters
Volume28
Issue number5
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Cytomegalovirus
  • Dual reporter gene
  • GFP-LacZ
  • Promoters
  • Stem cells tubulin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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