Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma

Jik Han Jung, Woojin Back, Junyong Yoon, Hyeonjeong Han, Ka Won Kang, Byeonghyeon Choi, Hyesun Jeong, Jaena Park, Hyunku Shin, Woojune Hur, Yeonho Choi, Sunghoi Hong, Hyun Koo Kim, Yong Park, Ji Ho Park

Research output: Contribution to journalArticlepeer-review

Abstract

Isolation of pure extracellular vesicles (EVs), especially from blood, has been a major challenge in the field of EV research. The presence of lipoproteins and soluble proteins often hinders the isolation of high purity EVs upon utilization of conventional separation methods. To circumvent such problems, we designed a single-step dual size-exclusion chromatography (dSEC) column for effective isolation of highly pure EVs from bone marrow derived human plasma. With an aim to select appropriate column design parameters, we analyzed the physiochemical properties of the major substances in bone marrow derived plasma, which include EVs, lipoproteins, and soluble proteins. Based on these findings, we devised a novel dSEC column with two different types of porous beads sequentially stacked each other for efficient separation of EVs from other contaminants. The newly developed dSEC columns exhibited better performance in isolating highly pure EVs from AML plasma in comparison to conventional isolation methods.

Original languageEnglish
Article number217
JournalScientific reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2021 Dec

ASJC Scopus subject areas

  • General

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