Dynamic Ligand Screening by Magnetic Nanoassembly Modulates Stem Cell Differentiation

Hyunsik Hong, Sunhong Min, Sagang Koo, Yunjung Lee, Jinho Yoon, Woo Young Jang, Nayeon Kang, Ramar Thangam, Hyojun Choi, Hee Joon Jung, Seong Beom Han, Qiang Wei, Seung Ho Yu, Dong Hwee Kim, Ramasamy Paulmurugan, Woong-Kyo Jeong, Ki Bum Lee, Taeghwan Hyeon, Dokyoon Kim, Heemin Kang

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In native microenvironment, diverse physical barriers exist to dynamically modulate stem cell recruitment and differentiation for tissue repair. In this study, nanoassembly-based magnetic screens of various sizes are utilized, and they are elastically tethered over an RGD ligand (cell-adhesive motif)-presenting material surface to generate various nanogaps between the screens and the RGDs without modulating the RGD density. Large screens exhibiting low RGD distribution stimulate integrin clustering to facilitate focal adhesion, mechanotransduction, and differentiation of stem cells, which are not observed with small screens. Magnetic downward pulling of the large screens decreases the nanogaps, which dynamically suppress the focal adhesion, mechanotransduction, and differentiation of stem cells. Conversely, magnetic upward pulling of the small screens increases the nanogaps, which dynamically activates focal adhesion, mechanotransduction, and differentiation of stem cells. This regulation mechanism is also shown to be effective in the microenvironment in vivo. Further diversifying the geometries of the physical screens can further enable diverse modalities of multifaceted and safe unscreening of the distributed RGDs to unravel and modulate stem cell differentiation for tissue repair.

Original languageEnglish
Article number2105460
JournalAdvanced Materials
Volume34
Issue number2
DOIs
Publication statusPublished - 2022 Jan 13

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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