Dynamic speckle illumination wide-field reflection phase microscopy

Youngwoon Choi, Poorya Hosseini, Wonshik Choi, Ramachandra R. Dasari, Peter T.C. So, Zahid Yaqoob

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We demonstrate a quantitative reflection-phase microscope based on time-varying speckle-field illumination. Due to the short spatial coherence length of the speckle field, the proposed imaging system features superior lateral resolution, 520 nm, as well as high-depth selectivity, 1.03 μm. Off-axis interferometric detection enables wide-field and single-shot imaging appropriate for high-speed measurements. In addition, the measured phase sensitivity of this method, which is the smallest measurable axial motion, is more than 40 times higher than that available using a transmission system. We demonstrate the utility of our method by successfully distinguishing the motion of the top surface from that of the bottom in red blood cells. The proposed method will be useful for studying membrane dynamics in complex eukaryotic cells.

Original languageEnglish
Pages (from-to)6062-6065
Number of pages4
JournalOptics Letters
Volume39
Issue number20
DOIs
Publication statusPublished - 2014 Oct 15

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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