E6 and E7 fusion immunoglobulin from human papilloma virus 16 induces dendritic cell maturation and antigen specific activation of T helper 1 response

Sang Hoon Kim, Yu Jin Hur, Suk Jun Lee, Sang Joon Kim, Chung Gyu Park, Yu Koung Oh, Woon Won Jung, Jong Bok Seo, Myung Hee Nam, Inho Choi, Taehoon Chun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human papilloma virus (HPV) 16 causes cervical cancer. Induction of oncogenesis by HPV 16 is primarily dependent on the function of E6 and E7 proteins, which inactivate the function of p53 and pRB, respectively. Thus, blocking the activity of the E6 and E7 proteins from HPV 16 is critical to inhibiting oncogenesis during infection. We have expressed and purified soluble HPV 16 E6 and E7 fusion immunoglobulin (Ig), which were combined with the constant region of an Ig heavy chain, in a mammalian system. To assess whether soluble E6 and E7 fusion Igs induce effective cellular immune responses, immature dendritic cells (DCs) were treated with these fusion proteins. Soluble E6 and E7 fusion Igs effectively induced maturation of DCs. Furthermore, immunization with soluble E6 and E7 fusion Igs in mice resulted in antigen-specific activation of T helper 1 (Th1) cells. This is the first comprehensive study to show the molecular basis of how soluble HPV 16 E6 or E7 fusion Igs induces Th1 responses through the maturation of DCs. In addition, we show that DC therapy using soluble HPV E6 and E7 fusion Igs may be a valuable tool for controlling the progress of cervical cancer.

Original languageEnglish
Pages (from-to)663-671
Number of pages9
JournalBiotechnology letters
Volume33
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

Keywords

  • Cervical cancer
  • Dendritic cell
  • Human papilloma virus 16
  • Oncogenesis
  • T cell activation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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