Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs

Je Hyeong Kim, Jeong Shim Jae Jeong Shim, Sung Yong Lee, Hwan Kwon Young Hwan Kwon, Ra Lee So Ra Lee, Youb Lee Sang Youb Lee, Youn Cho Jae Youn Cho, Ho In Kwang Ho In, Hwa Yoo Se Hwa Yoo, Ho Kang Kyung Ho Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Bronchial asthma is a complex disease, which is characterized by spontaneous exacerbations of airway obstruction and persistent bronchial hyperresponsiveness. Animal models have fallen short of reproducing the human disease, particularly in mimicking the spontaneous and persistent airflow obstruction that characterized in asthma. In animals, airflow obstruction is usually assessed by measuring airflow resistance during tidal breathing under such invasive technique as tracheostomy and anesthesia. A noninvasive technique for measuring pulmonary function in small animals is needed to evaluate long-term changes in lung function during the course of experimentally produced disease without sacrificing the animal. Purpose: The purpose of this study was to evaluate early bronchoconstriction after allergen challenge and airway responsiveness (AR) to inhaled methacholine in nonanesthetized, unrestrained guinea pigs. Method: Guinea pig model of asthma was sensitized by subcutaneous injection with ovalbumin and challenged by inhalation of aerosolized ovalbumin (1% wt/vol ovalbumin). Airflow obstruction of conscious guinea pig was measured as specific airway resistance (airway resistance x thoracic gas volume). Airway resistance and thoracic gas volume of conscious guinea pig were assessed by body plethysmography before challenge and at regular intervals for as long as 30 minutes after challenge. AR to aerosolized methacholine of asthma grou, was compared with that of control group in body plethysmography. Result: Asthma models developed in 13 (65%) among 20 guinea pigs, in which early responses occurred in airways after the exposure to inhalation with ovalbumin Airway challenge with ovalbumin caused increase in specific airway resistance, which peaked at 6 minutes and amounted to a 231.5 ± 30.4% increase from baseline, AR to aerosolized methacholine of asthma model increased significantly compared with control group. Conclusion: The results have showed a useful animal model to evaluate early bronchoconstriction after allergen challenge and airway responsiveness in nonanesthetized, unrestrained guinea pigs.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalTuberculosis and Respiratory Diseases
Volume45
Issue number1
Publication statusPublished - 1998 Jan 1

Fingerprint

Bronchoconstriction
Allergens
Ovalbumin
Guinea Pigs
Airway Resistance
Asthma
Methacholine Chloride
Plethysmography
Thorax
Animal Models
Gases
Inhalation Exposure
Lung
Control Groups
Tracheostomy
Airway Obstruction
Subcutaneous Injections
Inhalation
Respiration
Anesthesia

Keywords

  • Animal asthma model
  • Animal body plethysmography
  • Specific model airway resistance

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

Kim, J. H., Jae Jeong Shim, J. S., Lee, S. Y., Young Hwan Kwon, H. K., So Ra Lee, R. L., Sang Youb Lee, Y. L., ... Kyung Ho Kang, H. K. (1998). Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs. Tuberculosis and Respiratory Diseases, 45(1), 99-106.

Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs. / Kim, Je Hyeong; Jae Jeong Shim, Jeong Shim; Lee, Sung Yong; Young Hwan Kwon, Hwan Kwon; So Ra Lee, Ra Lee; Sang Youb Lee, Youb Lee; Jae Youn Cho, Youn Cho; Kwang Ho In, Ho In; Se Hwa Yoo, Hwa Yoo; Kyung Ho Kang, Ho Kang.

In: Tuberculosis and Respiratory Diseases, Vol. 45, No. 1, 01.01.1998, p. 99-106.

Research output: Contribution to journalArticle

Kim, JH, Jae Jeong Shim, JS, Lee, SY, Young Hwan Kwon, HK, So Ra Lee, RL, Sang Youb Lee, YL, Jae Youn Cho, YC, Kwang Ho In, HI, Se Hwa Yoo, HY & Kyung Ho Kang, HK 1998, 'Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs', Tuberculosis and Respiratory Diseases, vol. 45, no. 1, pp. 99-106.
Kim JH, Jae Jeong Shim JS, Lee SY, Young Hwan Kwon HK, So Ra Lee RL, Sang Youb Lee YL et al. Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs. Tuberculosis and Respiratory Diseases. 1998 Jan 1;45(1):99-106.
Kim, Je Hyeong ; Jae Jeong Shim, Jeong Shim ; Lee, Sung Yong ; Young Hwan Kwon, Hwan Kwon ; So Ra Lee, Ra Lee ; Sang Youb Lee, Youb Lee ; Jae Youn Cho, Youn Cho ; Kwang Ho In, Ho In ; Se Hwa Yoo, Hwa Yoo ; Kyung Ho Kang, Ho Kang. / Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs. In: Tuberculosis and Respiratory Diseases. 1998 ; Vol. 45, No. 1. pp. 99-106.
@article{1d4b79fb9b70491cadf006df0a471f12,
title = "Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs",
abstract = "Background: Bronchial asthma is a complex disease, which is characterized by spontaneous exacerbations of airway obstruction and persistent bronchial hyperresponsiveness. Animal models have fallen short of reproducing the human disease, particularly in mimicking the spontaneous and persistent airflow obstruction that characterized in asthma. In animals, airflow obstruction is usually assessed by measuring airflow resistance during tidal breathing under such invasive technique as tracheostomy and anesthesia. A noninvasive technique for measuring pulmonary function in small animals is needed to evaluate long-term changes in lung function during the course of experimentally produced disease without sacrificing the animal. Purpose: The purpose of this study was to evaluate early bronchoconstriction after allergen challenge and airway responsiveness (AR) to inhaled methacholine in nonanesthetized, unrestrained guinea pigs. Method: Guinea pig model of asthma was sensitized by subcutaneous injection with ovalbumin and challenged by inhalation of aerosolized ovalbumin (1{\%} wt/vol ovalbumin). Airflow obstruction of conscious guinea pig was measured as specific airway resistance (airway resistance x thoracic gas volume). Airway resistance and thoracic gas volume of conscious guinea pig were assessed by body plethysmography before challenge and at regular intervals for as long as 30 minutes after challenge. AR to aerosolized methacholine of asthma grou, was compared with that of control group in body plethysmography. Result: Asthma models developed in 13 (65{\%}) among 20 guinea pigs, in which early responses occurred in airways after the exposure to inhalation with ovalbumin Airway challenge with ovalbumin caused increase in specific airway resistance, which peaked at 6 minutes and amounted to a 231.5 ± 30.4{\%} increase from baseline, AR to aerosolized methacholine of asthma model increased significantly compared with control group. Conclusion: The results have showed a useful animal model to evaluate early bronchoconstriction after allergen challenge and airway responsiveness in nonanesthetized, unrestrained guinea pigs.",
keywords = "Animal asthma model, Animal body plethysmography, Specific model airway resistance",
author = "Kim, {Je Hyeong} and {Jae Jeong Shim}, {Jeong Shim} and Lee, {Sung Yong} and {Young Hwan Kwon}, {Hwan Kwon} and {So Ra Lee}, {Ra Lee} and {Sang Youb Lee}, {Youb Lee} and {Jae Youn Cho}, {Youn Cho} and {Kwang Ho In}, {Ho In} and {Se Hwa Yoo}, {Hwa Yoo} and {Kyung Ho Kang}, {Ho Kang}",
year = "1998",
month = "1",
day = "1",
language = "English",
volume = "45",
pages = "99--106",
journal = "Tuberculosis and Respiratory Diseases",
issn = "1738-3536",
publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",
number = "1",

}

TY - JOUR

T1 - Early bronchoconstriction after allergen challenge of nonanesthetized guinea pigs

AU - Kim, Je Hyeong

AU - Jae Jeong Shim, Jeong Shim

AU - Lee, Sung Yong

AU - Young Hwan Kwon, Hwan Kwon

AU - So Ra Lee, Ra Lee

AU - Sang Youb Lee, Youb Lee

AU - Jae Youn Cho, Youn Cho

AU - Kwang Ho In, Ho In

AU - Se Hwa Yoo, Hwa Yoo

AU - Kyung Ho Kang, Ho Kang

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Background: Bronchial asthma is a complex disease, which is characterized by spontaneous exacerbations of airway obstruction and persistent bronchial hyperresponsiveness. Animal models have fallen short of reproducing the human disease, particularly in mimicking the spontaneous and persistent airflow obstruction that characterized in asthma. In animals, airflow obstruction is usually assessed by measuring airflow resistance during tidal breathing under such invasive technique as tracheostomy and anesthesia. A noninvasive technique for measuring pulmonary function in small animals is needed to evaluate long-term changes in lung function during the course of experimentally produced disease without sacrificing the animal. Purpose: The purpose of this study was to evaluate early bronchoconstriction after allergen challenge and airway responsiveness (AR) to inhaled methacholine in nonanesthetized, unrestrained guinea pigs. Method: Guinea pig model of asthma was sensitized by subcutaneous injection with ovalbumin and challenged by inhalation of aerosolized ovalbumin (1% wt/vol ovalbumin). Airflow obstruction of conscious guinea pig was measured as specific airway resistance (airway resistance x thoracic gas volume). Airway resistance and thoracic gas volume of conscious guinea pig were assessed by body plethysmography before challenge and at regular intervals for as long as 30 minutes after challenge. AR to aerosolized methacholine of asthma grou, was compared with that of control group in body plethysmography. Result: Asthma models developed in 13 (65%) among 20 guinea pigs, in which early responses occurred in airways after the exposure to inhalation with ovalbumin Airway challenge with ovalbumin caused increase in specific airway resistance, which peaked at 6 minutes and amounted to a 231.5 ± 30.4% increase from baseline, AR to aerosolized methacholine of asthma model increased significantly compared with control group. Conclusion: The results have showed a useful animal model to evaluate early bronchoconstriction after allergen challenge and airway responsiveness in nonanesthetized, unrestrained guinea pigs.

AB - Background: Bronchial asthma is a complex disease, which is characterized by spontaneous exacerbations of airway obstruction and persistent bronchial hyperresponsiveness. Animal models have fallen short of reproducing the human disease, particularly in mimicking the spontaneous and persistent airflow obstruction that characterized in asthma. In animals, airflow obstruction is usually assessed by measuring airflow resistance during tidal breathing under such invasive technique as tracheostomy and anesthesia. A noninvasive technique for measuring pulmonary function in small animals is needed to evaluate long-term changes in lung function during the course of experimentally produced disease without sacrificing the animal. Purpose: The purpose of this study was to evaluate early bronchoconstriction after allergen challenge and airway responsiveness (AR) to inhaled methacholine in nonanesthetized, unrestrained guinea pigs. Method: Guinea pig model of asthma was sensitized by subcutaneous injection with ovalbumin and challenged by inhalation of aerosolized ovalbumin (1% wt/vol ovalbumin). Airflow obstruction of conscious guinea pig was measured as specific airway resistance (airway resistance x thoracic gas volume). Airway resistance and thoracic gas volume of conscious guinea pig were assessed by body plethysmography before challenge and at regular intervals for as long as 30 minutes after challenge. AR to aerosolized methacholine of asthma grou, was compared with that of control group in body plethysmography. Result: Asthma models developed in 13 (65%) among 20 guinea pigs, in which early responses occurred in airways after the exposure to inhalation with ovalbumin Airway challenge with ovalbumin caused increase in specific airway resistance, which peaked at 6 minutes and amounted to a 231.5 ± 30.4% increase from baseline, AR to aerosolized methacholine of asthma model increased significantly compared with control group. Conclusion: The results have showed a useful animal model to evaluate early bronchoconstriction after allergen challenge and airway responsiveness in nonanesthetized, unrestrained guinea pigs.

KW - Animal asthma model

KW - Animal body plethysmography

KW - Specific model airway resistance

UR - http://www.scopus.com/inward/record.url?scp=0031863968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031863968&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031863968

VL - 45

SP - 99

EP - 106

JO - Tuberculosis and Respiratory Diseases

JF - Tuberculosis and Respiratory Diseases

SN - 1738-3536

IS - 1

ER -