Early detection of cysteine rich protein 61 (CYR61, CCN1) in urine following renal ischemic reperfusion injury

Yasunar Muramatsu, Michiko Tsujie, Yukimasa Kohda, Bertha Pham, Alan O. Perantoni, Hong Zhao, Sang Kyung Jo, Peter S.T. Yuen, Leonard Craig, Xuzhen Hu, Robert A. Star

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Background. Acute renal failure (ARF) has a high morbidity and mortality. Many therapies have worked in animals but were unsuccessful in clinical trials. The inability to diagnose ARF early may have impaired the success of these trials. Method. We screened a subtraction library to search for potential disease markers that would be induced rapidly after renal injury. Mice and rats were subjected to 30 to 40 minutes of bilateral ischemia. Results. mRNA for Cyr61, a secreted growth factor-inducible immediate early gene, was markedly up-regulated at two hours in the kidney but not other organs following renal ischemia. In situ hybridization studies suggested Cyr61 was synthesized in the proximal straight tubule. Cyr61 protein was analyzed by capture with heparin beads followed by Western blotting. Induction of Cyr61 protein could be detected in the kidney within one hour, peaked at four to eight hours, and remained elevated for at least 24 hours following ischemia. Cyr61 protein was detected in urine at three to six hours and peaked at six to nine hours after renal injury. Cyr61 was not detected after volume depletion, which is often difficult to differentiate from ARF. Conclusions. The secreted, cysteine-rich, heparin binding protein Cyr61 is rapidly induced in proximal straight tubules following renal ischemia, and excreted in the urine where it might serve as an early biomarker of renal injury.

Original languageEnglish
Pages (from-to)1601-1610
Number of pages10
JournalKidney International
Volume62
Issue number5
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Fingerprint

Cysteine-Rich Protein 61
Reperfusion Injury
Urine
Kidney
Ischemia
Acute Kidney Injury
Wounds and Injuries
Proteins
Immediate-Early Genes
Libraries
In Situ Hybridization
Cysteine
Heparin
Intercellular Signaling Peptides and Proteins
Biomarkers
Western Blotting
Clinical Trials
Morbidity
Messenger RNA

Keywords

  • Biomarker
  • Cyr61
  • Ischemia
  • Kidney injury
  • Reperfusion

ASJC Scopus subject areas

  • Nephrology

Cite this

Muramatsu, Y., Tsujie, M., Kohda, Y., Pham, B., Perantoni, A. O., Zhao, H., ... Star, R. A. (2002). Early detection of cysteine rich protein 61 (CYR61, CCN1) in urine following renal ischemic reperfusion injury. Kidney International, 62(5), 1601-1610. https://doi.org/10.1046/j.1523-1755.2002.00633.x

Early detection of cysteine rich protein 61 (CYR61, CCN1) in urine following renal ischemic reperfusion injury. / Muramatsu, Yasunar; Tsujie, Michiko; Kohda, Yukimasa; Pham, Bertha; Perantoni, Alan O.; Zhao, Hong; Jo, Sang Kyung; Yuen, Peter S.T.; Craig, Leonard; Hu, Xuzhen; Star, Robert A.

In: Kidney International, Vol. 62, No. 5, 01.01.2002, p. 1601-1610.

Research output: Contribution to journalArticle

Muramatsu, Y, Tsujie, M, Kohda, Y, Pham, B, Perantoni, AO, Zhao, H, Jo, SK, Yuen, PST, Craig, L, Hu, X & Star, RA 2002, 'Early detection of cysteine rich protein 61 (CYR61, CCN1) in urine following renal ischemic reperfusion injury', Kidney International, vol. 62, no. 5, pp. 1601-1610. https://doi.org/10.1046/j.1523-1755.2002.00633.x
Muramatsu, Yasunar ; Tsujie, Michiko ; Kohda, Yukimasa ; Pham, Bertha ; Perantoni, Alan O. ; Zhao, Hong ; Jo, Sang Kyung ; Yuen, Peter S.T. ; Craig, Leonard ; Hu, Xuzhen ; Star, Robert A. / Early detection of cysteine rich protein 61 (CYR61, CCN1) in urine following renal ischemic reperfusion injury. In: Kidney International. 2002 ; Vol. 62, No. 5. pp. 1601-1610.
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AU - Tsujie, Michiko

AU - Kohda, Yukimasa

AU - Pham, Bertha

AU - Perantoni, Alan O.

AU - Zhao, Hong

AU - Jo, Sang Kyung

AU - Yuen, Peter S.T.

AU - Craig, Leonard

AU - Hu, Xuzhen

AU - Star, Robert A.

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