Early effects of PP60(v-src) kinase activation on caveolae

Young Gyu Ko, Pingsheng Liu, Ravindra K. Pathak, Leonard C. Craig, Richard G.W. Anderson

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Members of the nonreceptor tyrosine kinase family appear to be targeted to caveolae membrane. We have used a Rat-1 cell expressing a temperature sensitive pp60(v-src) kinase to assess the initial changes that take place in caveolae after kinase activation. Within 24-48 h after cells were shifted to the permissive temperature, a set of caveolae-specific proteins became phosphorylated on tyrosine. During this period there was a decline in the caveolae marker protein, caveolin-1, a loss of invaginated caveolae, and a 70% decline in the sphingomyelin content of the cell. One of the phosphorylated proteins was caveolin-1 but it was associated in coimmunoprecipitation assays with both a 30 kDa and a 27 kDa tyrosine- phosphorylated protein. Finally, the cells changed from having a typical fibroblast morphology to a rounded shape lacking polarity. In light of the recent evidence that diverse signaling events originate from caveolae, pp60 (v-src) kinase appears to cause global changes to this membrane domain that might directly contribute to the transformed phenotype.

Original languageEnglish
Pages (from-to)524-535
Number of pages12
JournalJournal of cellular biochemistry
Volume71
Issue number4
DOIs
Publication statusPublished - 1998 Dec 15

Keywords

  • Caveolae
  • Caveolin-1
  • Cell transformation
  • Tyrosine kinase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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