Abstract
BACKGROUND: Sepsis is a medical emergency during which early detection is closely associated with mortality. In sepsis, red blood cell (RBC) abnormalities have been reported. However, it is not known how early RBC abnormalities are expressed compared with various clinical manifestations used in sepsis-related organ failure assessment (SOFA). OBJECTIVE: Therefore, using a lipopolysaccharide (LPS)-induced sepsis model we investigated the clinical significance of RBC abnormalities as an early indicator in the detection of septic injury compared with clinical variables. METHODS: Sprague-Dawley rats received LPS (20 mg/kg) intraperitoneally. Aggregation indices (AIs) and aggregation half-time (T1/2), and elongation indices (EI max) were measured. Clinical data-related SOFA and lactate were measured at 2 h, 4 h, 8 h and 12 h after LPS injection. RESULTS: AIs increased at 4 h, and T1/2 decreased at 2 h after LPS injection. Platelet counts decreased at 4 h, and lactate increased at 2 h after LPS injection. AIs showed strong correlations with T1/2 and platelets, EI max increased at 2 h after LPS injection, while EI max had a positive correlation with lactate. CONCLUSIONS: RBC aggregation appears to be an early indicator of clinical deterioration in sepsis and may represent a diagnostic indicator in sepsis.
| Original language | English |
|---|---|
| Pages (from-to) | 355-363 |
| Number of pages | 9 |
| Journal | Clinical Hemorheology and Microcirculation |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2018 Jan 1 |
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Keywords
- Red blood cell aggregation
- red blood cell deformability
- sepsis
- sepsis-related organ failure assessment
ASJC Scopus subject areas
- Physiology
- Hematology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
Early red blood cell abnormalities as a clinical variable in sepsis diagnosis. / Ko, Eunji; Youn, Jung Min; Park, Hyung Sun; Song, Myeongjin; Koh, Kyung Hee; Lim, Choon Hak.
In: Clinical Hemorheology and Microcirculation, Vol. 70, No. 3, 01.01.2018, p. 355-363.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early red blood cell abnormalities as a clinical variable in sepsis diagnosis
AU - Ko, Eunji
AU - Youn, Jung Min
AU - Park, Hyung Sun
AU - Song, Myeongjin
AU - Koh, Kyung Hee
AU - Lim, Choon Hak
PY - 2018/1/1
Y1 - 2018/1/1
N2 - BACKGROUND: Sepsis is a medical emergency during which early detection is closely associated with mortality. In sepsis, red blood cell (RBC) abnormalities have been reported. However, it is not known how early RBC abnormalities are expressed compared with various clinical manifestations used in sepsis-related organ failure assessment (SOFA). OBJECTIVE: Therefore, using a lipopolysaccharide (LPS)-induced sepsis model we investigated the clinical significance of RBC abnormalities as an early indicator in the detection of septic injury compared with clinical variables. METHODS: Sprague-Dawley rats received LPS (20 mg/kg) intraperitoneally. Aggregation indices (AIs) and aggregation half-time (T1/2), and elongation indices (EI max) were measured. Clinical data-related SOFA and lactate were measured at 2 h, 4 h, 8 h and 12 h after LPS injection. RESULTS: AIs increased at 4 h, and T1/2 decreased at 2 h after LPS injection. Platelet counts decreased at 4 h, and lactate increased at 2 h after LPS injection. AIs showed strong correlations with T1/2 and platelets, EI max increased at 2 h after LPS injection, while EI max had a positive correlation with lactate. CONCLUSIONS: RBC aggregation appears to be an early indicator of clinical deterioration in sepsis and may represent a diagnostic indicator in sepsis.
AB - BACKGROUND: Sepsis is a medical emergency during which early detection is closely associated with mortality. In sepsis, red blood cell (RBC) abnormalities have been reported. However, it is not known how early RBC abnormalities are expressed compared with various clinical manifestations used in sepsis-related organ failure assessment (SOFA). OBJECTIVE: Therefore, using a lipopolysaccharide (LPS)-induced sepsis model we investigated the clinical significance of RBC abnormalities as an early indicator in the detection of septic injury compared with clinical variables. METHODS: Sprague-Dawley rats received LPS (20 mg/kg) intraperitoneally. Aggregation indices (AIs) and aggregation half-time (T1/2), and elongation indices (EI max) were measured. Clinical data-related SOFA and lactate were measured at 2 h, 4 h, 8 h and 12 h after LPS injection. RESULTS: AIs increased at 4 h, and T1/2 decreased at 2 h after LPS injection. Platelet counts decreased at 4 h, and lactate increased at 2 h after LPS injection. AIs showed strong correlations with T1/2 and platelets, EI max increased at 2 h after LPS injection, while EI max had a positive correlation with lactate. CONCLUSIONS: RBC aggregation appears to be an early indicator of clinical deterioration in sepsis and may represent a diagnostic indicator in sepsis.
KW - Red blood cell aggregation
KW - red blood cell deformability
KW - sepsis
KW - sepsis-related organ failure assessment
UR - http://www.scopus.com/inward/record.url?scp=85057812556&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85057812556&partnerID=8YFLogxK
U2 - 10.3233/CH-180430
DO - 10.3233/CH-180430
M3 - Article
C2 - 30320561
AN - SCOPUS:85057812556
VL - 70
SP - 355
EP - 363
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
SN - 1386-0291
IS - 3
ER -