Ecdysone-responsive microRNA-252-5p controls the cell cycle by targeting Abi in Drosophila

Do Hwan Lim, Seungjae Lee, Jee Yun Han, Min Seok Choi, Jae Sang Hong, Youngmo Seong, Young Soo Kwon, Youngsik Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The steroid hormone ecdysone has a central role in the developmental transitions of insects through its control of responsive protein-coding and microRNA (miRNA) gene expression. However, the complete regulatory network controlling the expression of these genes remains to be elucidated. In this study, we performed cross-linking immunoprecipitation coupled with deep sequencing of endogenous Argonaute 1 (Ago1) protein, the core effector of the miRNA pathway, in Drosophila S2 cells. We found that regulatory interactions between miRNAs and their cognate targets were substantially altered by Ago1 in response to ecdysone signaling. Additionally, during the larva-to-adult metamorphosis, miR-252-5p was up-regulated via the canonical ecdysone-signaling pathway. Moreover, we provide evidence that miR-252-5p targets Abelson interacting protein (Abi) to decrease the protein levels of cyclins A and B, controlling the cell cycle. Overall, our data suggest a potential role for the ecdysone/miR-252-5p/Abi regulatory axis partly in cell-cycle control during metamorphosis in Drosophila.

Original languageEnglish
Pages (from-to)4519-4533
Number of pages15
JournalFASEB Journal
Volume32
Issue number8
DOIs
Publication statusPublished - 2018 Aug 1

Fingerprint

Ecdysone
Cell Cycle Checkpoints
MicroRNAs
Cells
Drosophila
Proteins
Argonaute Proteins
Cyclin B
Gene Expression
High-Throughput Nucleotide Sequencing
Cyclin A
Steroid hormones
Immunoprecipitation
Larva
Insects
Cell Cycle
Gene expression
Steroids
Hormones
Genes

Keywords

  • 20E
  • Ago1
  • CLIP-seq
  • Cyclin
  • MicroRNA

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Ecdysone-responsive microRNA-252-5p controls the cell cycle by targeting Abi in Drosophila. / Lim, Do Hwan; Lee, Seungjae; Han, Jee Yun; Choi, Min Seok; Hong, Jae Sang; Seong, Youngmo; Kwon, Young Soo; Lee, Youngsik.

In: FASEB Journal, Vol. 32, No. 8, 01.08.2018, p. 4519-4533.

Research output: Contribution to journalArticle

Lim, DH, Lee, S, Han, JY, Choi, MS, Hong, JS, Seong, Y, Kwon, YS & Lee, Y 2018, 'Ecdysone-responsive microRNA-252-5p controls the cell cycle by targeting Abi in Drosophila', FASEB Journal, vol. 32, no. 8, pp. 4519-4533. https://doi.org/10.1096/fj.201701185RR
Lim, Do Hwan ; Lee, Seungjae ; Han, Jee Yun ; Choi, Min Seok ; Hong, Jae Sang ; Seong, Youngmo ; Kwon, Young Soo ; Lee, Youngsik. / Ecdysone-responsive microRNA-252-5p controls the cell cycle by targeting Abi in Drosophila. In: FASEB Journal. 2018 ; Vol. 32, No. 8. pp. 4519-4533.
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