TY - JOUR
T1 - Echinicola sediminis sp. Nov., a marine bacterium isolated from coastal sediment
AU - Lee, Dong Wan
AU - Lee, Aslan Hwanhwi
AU - Lee, Hanbyul
AU - Kim, Jae Jin
AU - Khim, Jong Seong
AU - Yim, Un Hyuk
AU - Kim, Beom Seok
N1 - Funding Information:
This work was supported by the project entitled ‘Oil Spill Environmental Impact Assessment and Environmental Restoration (PM57431)’ funded by the Ministry of Oceans and Fisheries of Korea, the BK21 Plus program in 2013 (Project No. 21A20130012270) funded by the National Research Foundation of Korea (NRF) and a Korea University Grant.
Publisher Copyright:
© 2017 IUMS.
PY - 2017/9
Y1 - 2017/9
N2 - A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were ‘Echinicola shivajiensis’ AK12T (98.1% 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754T (96.4 %), Echinicola jeungdonensis HMD 3054T (96.0 %), Echinicola pacifica KMM 6172T (96.0 %) and Echinicola rosea JL 3085T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15: 0, summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C17: 1ω6c and summed feature 9 (comprising iso-C17: 1ω9c and/or 10-methyl C16: 0). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA– DNA relatedness between strain 001-Na2T and ‘E. shivajiensis’ JCM 17847T was 20.8–21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA–DNA hybridization analyses presented, strain 001-Na2T (=KCTC 52495T=JCM 31786T =DSM 103729T) is the type strain of a novel species of the genus Echinicola, for which the name Echinicola sediminis sp. nov. is proposed.
AB - A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were ‘Echinicola shivajiensis’ AK12T (98.1% 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754T (96.4 %), Echinicola jeungdonensis HMD 3054T (96.0 %), Echinicola pacifica KMM 6172T (96.0 %) and Echinicola rosea JL 3085T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15: 0, summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C17: 1ω6c and summed feature 9 (comprising iso-C17: 1ω9c and/or 10-methyl C16: 0). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA– DNA relatedness between strain 001-Na2T and ‘E. shivajiensis’ JCM 17847T was 20.8–21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA–DNA hybridization analyses presented, strain 001-Na2T (=KCTC 52495T=JCM 31786T =DSM 103729T) is the type strain of a novel species of the genus Echinicola, for which the name Echinicola sediminis sp. nov. is proposed.
KW - Bacteroidetes
KW - Coastal sediment
KW - Echinicola
KW - Marine bacterium
UR - http://www.scopus.com/inward/record.url?scp=85029603942&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002118
DO - 10.1099/ijsem.0.002118
M3 - Article
C2 - 28857020
AN - SCOPUS:85029603942
SN - 1466-5026
VL - 67
SP - 3351
EP - 3357
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 9
M1 - 002118
ER -
