Echogenic glycol chitosan nanoparticles for ultrasound-triggered cancer theranostics

Hyun Su Min, Dong Gil You, Sejin Son, Sangmin Jeon, Jae Hyung Park, Seulki Lee, Ick Chan Kwon, Kwang Meyung Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Theranostic nanoparticles hold great promise for simultaneous diagnosis of diseases, targeted drug delivery with minimal toxicity, and monitoring of therapeutic efficacy. However, one of the current challenges in developing theranostic nanoparticles is enhancing the tumor-specific targeting of both imaging probes and anticancer agents. Herein, we report the development of tumor-homing echogenic glycol chitosan-based nanoparticles (Echo-CNPs) that concurrently execute cancer- targeted ultrasound (US) imaging and US-triggered drug delivery. To construct this novel Echo-CNPs, an anticancer drug and bioinert perfluoropentane (PFP), a US gas precursor, were simultaneously encapsulated into glycol chitosan nanoparticles using the oil in water (O/W) emulsion method. The resulting Echo-CNPs had a nano-sized particle structure, composing of hydrophobic anticancer drug/PFP inner cores and a hydrophilic glycol chitosan polymer outer shell. The Echo-CNPs had a favorable hydrodynamic size of 432 nm, which is entirely different from the micro-sized core-empty conventional microbubbles (1-10 μm). Furthermore, Echo-CNPs showed the prolonged echogenicity via the sustained microbubble formation process of liquid-phase PFP at the body temperature and they also presented a US-triggered drug release profile through the external US irradiation. Interestingly, Echo-CNPs exhibited significantly increased tumor-homing ability with lower non-specific uptake by other tissues in tumor-bearing mice through the nanoparticle's enhanced permeation and retention (EPR) effect. Conclusively, theranostic Echo-CNPs are highly useful for simultaneous cancer-targeting US imaging and US-triggered delivery in cancer theranostics.

Original languageEnglish
Pages (from-to)1402-1418
Number of pages17
JournalTheranostics
Volume5
Issue number12
DOIs
Publication statusPublished - 2015

Fingerprint

Nanoparticles
Neoplasms
Microbubbles
Pharmaceutical Preparations
Ultrasonography
Theranostic Nanomedicine
glycol-chitosan
Hydrodynamics
Emulsions
Body Temperature
Antineoplastic Agents
Polymers
Oils
Gases
Water

Keywords

  • Drug delivery
  • Echogenicity
  • Theranostic nanoparticle
  • Tumor targeting
  • Ultrasound contrast agent
  • Ultrasound imaging

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Cite this

Echogenic glycol chitosan nanoparticles for ultrasound-triggered cancer theranostics. / Min, Hyun Su; You, Dong Gil; Son, Sejin; Jeon, Sangmin; Park, Jae Hyung; Lee, Seulki; Kwon, Ick Chan; Kim, Kwang Meyung.

In: Theranostics, Vol. 5, No. 12, 2015, p. 1402-1418.

Research output: Contribution to journalArticle

Min, HS, You, DG, Son, S, Jeon, S, Park, JH, Lee, S, Kwon, IC & Kim, KM 2015, 'Echogenic glycol chitosan nanoparticles for ultrasound-triggered cancer theranostics', Theranostics, vol. 5, no. 12, pp. 1402-1418. https://doi.org/10.7150/thno.13099
Min, Hyun Su ; You, Dong Gil ; Son, Sejin ; Jeon, Sangmin ; Park, Jae Hyung ; Lee, Seulki ; Kwon, Ick Chan ; Kim, Kwang Meyung. / Echogenic glycol chitosan nanoparticles for ultrasound-triggered cancer theranostics. In: Theranostics. 2015 ; Vol. 5, No. 12. pp. 1402-1418.
@article{b82942a5a7c540dda3dcd434f1269036,
title = "Echogenic glycol chitosan nanoparticles for ultrasound-triggered cancer theranostics",
abstract = "Theranostic nanoparticles hold great promise for simultaneous diagnosis of diseases, targeted drug delivery with minimal toxicity, and monitoring of therapeutic efficacy. However, one of the current challenges in developing theranostic nanoparticles is enhancing the tumor-specific targeting of both imaging probes and anticancer agents. Herein, we report the development of tumor-homing echogenic glycol chitosan-based nanoparticles (Echo-CNPs) that concurrently execute cancer- targeted ultrasound (US) imaging and US-triggered drug delivery. To construct this novel Echo-CNPs, an anticancer drug and bioinert perfluoropentane (PFP), a US gas precursor, were simultaneously encapsulated into glycol chitosan nanoparticles using the oil in water (O/W) emulsion method. The resulting Echo-CNPs had a nano-sized particle structure, composing of hydrophobic anticancer drug/PFP inner cores and a hydrophilic glycol chitosan polymer outer shell. The Echo-CNPs had a favorable hydrodynamic size of 432 nm, which is entirely different from the micro-sized core-empty conventional microbubbles (1-10 μm). Furthermore, Echo-CNPs showed the prolonged echogenicity via the sustained microbubble formation process of liquid-phase PFP at the body temperature and they also presented a US-triggered drug release profile through the external US irradiation. Interestingly, Echo-CNPs exhibited significantly increased tumor-homing ability with lower non-specific uptake by other tissues in tumor-bearing mice through the nanoparticle's enhanced permeation and retention (EPR) effect. Conclusively, theranostic Echo-CNPs are highly useful for simultaneous cancer-targeting US imaging and US-triggered delivery in cancer theranostics.",
keywords = "Drug delivery, Echogenicity, Theranostic nanoparticle, Tumor targeting, Ultrasound contrast agent, Ultrasound imaging",
author = "Min, {Hyun Su} and You, {Dong Gil} and Sejin Son and Sangmin Jeon and Park, {Jae Hyung} and Seulki Lee and Kwon, {Ick Chan} and Kim, {Kwang Meyung}",
year = "2015",
doi = "10.7150/thno.13099",
language = "English",
volume = "5",
pages = "1402--1418",
journal = "Theranostics",
issn = "1838-7640",
publisher = "Ivyspring International Publisher",
number = "12",

}

TY - JOUR

T1 - Echogenic glycol chitosan nanoparticles for ultrasound-triggered cancer theranostics

AU - Min, Hyun Su

AU - You, Dong Gil

AU - Son, Sejin

AU - Jeon, Sangmin

AU - Park, Jae Hyung

AU - Lee, Seulki

AU - Kwon, Ick Chan

AU - Kim, Kwang Meyung

PY - 2015

Y1 - 2015

N2 - Theranostic nanoparticles hold great promise for simultaneous diagnosis of diseases, targeted drug delivery with minimal toxicity, and monitoring of therapeutic efficacy. However, one of the current challenges in developing theranostic nanoparticles is enhancing the tumor-specific targeting of both imaging probes and anticancer agents. Herein, we report the development of tumor-homing echogenic glycol chitosan-based nanoparticles (Echo-CNPs) that concurrently execute cancer- targeted ultrasound (US) imaging and US-triggered drug delivery. To construct this novel Echo-CNPs, an anticancer drug and bioinert perfluoropentane (PFP), a US gas precursor, were simultaneously encapsulated into glycol chitosan nanoparticles using the oil in water (O/W) emulsion method. The resulting Echo-CNPs had a nano-sized particle structure, composing of hydrophobic anticancer drug/PFP inner cores and a hydrophilic glycol chitosan polymer outer shell. The Echo-CNPs had a favorable hydrodynamic size of 432 nm, which is entirely different from the micro-sized core-empty conventional microbubbles (1-10 μm). Furthermore, Echo-CNPs showed the prolonged echogenicity via the sustained microbubble formation process of liquid-phase PFP at the body temperature and they also presented a US-triggered drug release profile through the external US irradiation. Interestingly, Echo-CNPs exhibited significantly increased tumor-homing ability with lower non-specific uptake by other tissues in tumor-bearing mice through the nanoparticle's enhanced permeation and retention (EPR) effect. Conclusively, theranostic Echo-CNPs are highly useful for simultaneous cancer-targeting US imaging and US-triggered delivery in cancer theranostics.

AB - Theranostic nanoparticles hold great promise for simultaneous diagnosis of diseases, targeted drug delivery with minimal toxicity, and monitoring of therapeutic efficacy. However, one of the current challenges in developing theranostic nanoparticles is enhancing the tumor-specific targeting of both imaging probes and anticancer agents. Herein, we report the development of tumor-homing echogenic glycol chitosan-based nanoparticles (Echo-CNPs) that concurrently execute cancer- targeted ultrasound (US) imaging and US-triggered drug delivery. To construct this novel Echo-CNPs, an anticancer drug and bioinert perfluoropentane (PFP), a US gas precursor, were simultaneously encapsulated into glycol chitosan nanoparticles using the oil in water (O/W) emulsion method. The resulting Echo-CNPs had a nano-sized particle structure, composing of hydrophobic anticancer drug/PFP inner cores and a hydrophilic glycol chitosan polymer outer shell. The Echo-CNPs had a favorable hydrodynamic size of 432 nm, which is entirely different from the micro-sized core-empty conventional microbubbles (1-10 μm). Furthermore, Echo-CNPs showed the prolonged echogenicity via the sustained microbubble formation process of liquid-phase PFP at the body temperature and they also presented a US-triggered drug release profile through the external US irradiation. Interestingly, Echo-CNPs exhibited significantly increased tumor-homing ability with lower non-specific uptake by other tissues in tumor-bearing mice through the nanoparticle's enhanced permeation and retention (EPR) effect. Conclusively, theranostic Echo-CNPs are highly useful for simultaneous cancer-targeting US imaging and US-triggered delivery in cancer theranostics.

KW - Drug delivery

KW - Echogenicity

KW - Theranostic nanoparticle

KW - Tumor targeting

KW - Ultrasound contrast agent

KW - Ultrasound imaging

UR - http://www.scopus.com/inward/record.url?scp=84954214760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954214760&partnerID=8YFLogxK

U2 - 10.7150/thno.13099

DO - 10.7150/thno.13099

M3 - Article

C2 - 26681985

AN - SCOPUS:84954214760

VL - 5

SP - 1402

EP - 1418

JO - Theranostics

JF - Theranostics

SN - 1838-7640

IS - 12

ER -