Eco-friendly composite of Fe3O4-reduced graphene oxide particles for efficient enzyme immobilization

Sanjay K.S. Patel, Seung Ho Choi, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A novel type of spherical and porous composites were synthesized to dually benefit from reduced graphene oxide (rGO) and magnetic materials as supports for enzyme immobilization. Three magnetic composite particles of Fe3O4 and rGO containing 71% (rGO-Fe3O4-M1), 36% (rGO-Fe3O4-M2), and 18% (rGO-Fe3O4-M3) Fe were prepared using a one-pot spray pyrolysis method and were used for the immobilization of the model enzymes, laccase and horseradish peroxidase (HRP). The rGO-Fe3O4 composite particles prepared by spray pyrolysis process had a regular shape, finite size, and uniform composition. The immobilization of laccase and HRP on rGO-Fe3O4-M1 resulted in 112 and 89.8% immobilization efficiency higher than that of synthesized pure Fe3O4 and rGO particles, respectively. The stability of laccase was improved by approximately 15-fold at 25 °C. Furthermore, rGO-Fe3O4-M1-immobilized laccase exhibited 92.6% of residual activity after 10 cycles of reuse and was 192% more efficient in oxidizing different phenolic compounds than the free enzyme. Therefore, these unique composite particles containing rGO and Fe3O4 may be promising supports for the efficient immobilization of industrially important enzymes with lower acute toxicity toward Vibrio f ischeri than commercial pure Fe3O4 particles.

Original languageEnglish
Pages (from-to)2213-2222
Number of pages10
JournalACS Applied Materials and Interfaces
Volume9
Issue number3
DOIs
Publication statusPublished - 2017 Jan 25

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Enzyme immobilization
Graphite
Oxides
Graphene
Laccase
Composite materials
Spray pyrolysis
Enzymes
Horseradish Peroxidase
Magnetic materials
Catalyst supports
Toxicity

Keywords

  • Acute Toxicity
  • Immobilization
  • Laccase
  • Magnetic Composite Particle
  • One-Pot Synthesis
  • Stability

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Eco-friendly composite of Fe3O4-reduced graphene oxide particles for efficient enzyme immobilization. / Patel, Sanjay K.S.; Choi, Seung Ho; Kang, Yun Chan; Lee, Jung Kul.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 3, 25.01.2017, p. 2213-2222.

Research output: Contribution to journalArticle

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