Ectopic expression of Tsi1 in transgenic hot pepper plants enhances host resistance to viral, bacterial, and oomycete pathogens

Ryoung Shin, Jeong Mee Park, Jong M. An, Kyung Hee Paek

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

In many plants, including hot pepper plants, productivity is greatly affected by pathogen attack. We reported previously that tobacco stress-induced gene 1 (Tsi1) may play an important role in regulating stress responsive genes and pathogenesis-related (PR) genes. In this study, we demonstrated that overexpression of Tsi1 gene in transgenic hot pepper plants induced constitutive expression of several PR genes in the absence of stress or pathogen treatment. The transgenic hot pepper plants expressing Tsi1 exhibited resistance to Pepper mild mottle virus (PMMV) and Cucumber mosaic virus (CMV). Furthermore, these transgenic plants showed increased resistance to a bacterial pathogen, Xanthomonas campestris pv. vesicatoria and also an oomycete pathogen, Phytophthora capsici. These results suggested that ectopic expression of Tsi1 in transgenic hot pepper plants enhanced the resistance of the plants to various pathogens, including viruses, bacteria, and oomycete. These results suggest that using transcriptional regulatory protein genes may contribute to developing broad-spectrum resistance in crop plants.

Original languageEnglish
Pages (from-to)983-989
Number of pages7
JournalMolecular Plant-Microbe Interactions
Volume15
Issue number10
Publication statusPublished - 2002 Oct 1

Fingerprint

Oomycetes
Capsicum
hot peppers
Tobacco
Pathogens
tobacco
host plants
Genes
genetically modified organisms
pathogens
genes
Viruses
Cucumovirus
Xanthomonas campestris
Xanthomonas vesicatoria
pathogenesis
Pepper mild mottle virus
Phytophthora
Ectopic Gene Expression
Genetically Modified Plants

Keywords

  • Pathogen resistance
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Microbiology

Cite this

Ectopic expression of Tsi1 in transgenic hot pepper plants enhances host resistance to viral, bacterial, and oomycete pathogens. / Shin, Ryoung; Park, Jeong Mee; An, Jong M.; Paek, Kyung Hee.

In: Molecular Plant-Microbe Interactions, Vol. 15, No. 10, 01.10.2002, p. 983-989.

Research output: Contribution to journalArticle

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