Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection

Hong Soon Yim, Kyung Min Choi, Byoungjae Kim, In Duk Jung, Yeong Min Park, Yoon Kyu Kang, Min Goo Lee

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and greater consistency. Administration of 1-methyl-D-tryptophan (1-MT) to inhibit indoleamine 2,3-dioxygenase (IDO) in mice with CCI-induced sepsis increased the survival rate and tended to up-regulate IL-10/IL-12 serum concentrations. The effectiveness of 1-MT was confirmed by increases in IL-10 over IL-12 in bone marrow-derived dendritic cells (BMDCs) treated with LPS and 1-MT and a superior survival rate 24 hr after injection of these double treated BMDCs in the CCI-induced sepsis model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis. The present findings using this newly developed model suggest that inhibition of IDO alleviates the severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic septic inflammation.

    Original languageEnglish
    Pages (from-to)633-639
    Number of pages7
    JournalMicrobiology and Immunology
    Volume57
    Issue number9
    DOIs
    Publication statusPublished - 2013 Sep

    Keywords

    • Clinical immunology
    • Dendritic cells
    • Innate immunity

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Virology

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