Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection

Hong Soon Yim, Kyung Min Choi, Byoungjae Kim, In Duk Jung, Yeong Min Park, Yoon Kyoo Kang, Min-Goo Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and greater consistency. Administration of 1-methyl-D-tryptophan (1-MT) to inhibit indoleamine 2,3-dioxygenase (IDO) in mice with CCI-induced sepsis increased the survival rate and tended to up-regulate IL-10/IL-12 serum concentrations. The effectiveness of 1-MT was confirmed by increases in IL-10 over IL-12 in bone marrow-derived dendritic cells (BMDCs) treated with LPS and 1-MT and a superior survival rate 24 hr after injection of these double treated BMDCs in the CCI-induced sepsis model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis. The present findings using this newly developed model suggest that inhibition of IDO alleviates the severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic septic inflammation.

Original languageEnglish
Pages (from-to)633-639
Number of pages7
JournalMicrobiology and Immunology
Volume57
Issue number9
DOIs
Publication statusPublished - 2013 Sep 1

Fingerprint

Adoptive Transfer
Dendritic Cells
Sepsis
Injections
Indoleamine-Pyrrole 2,3,-Dioxygenase
Interleukin-12
Interleukin-10
Bone Marrow
tryptophan methyl ester
Up-Regulation
Inflammation
Serum

Keywords

  • Clinical immunology
  • Dendritic cells
  • Innate immunity

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection. / Yim, Hong Soon; Choi, Kyung Min; Kim, Byoungjae; Jung, In Duk; Park, Yeong Min; Kang, Yoon Kyoo; Lee, Min-Goo.

In: Microbiology and Immunology, Vol. 57, No. 9, 01.09.2013, p. 633-639.

Research output: Contribution to journalArticle

Yim, Hong Soon ; Choi, Kyung Min ; Kim, Byoungjae ; Jung, In Duk ; Park, Yeong Min ; Kang, Yoon Kyoo ; Lee, Min-Goo. / Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection. In: Microbiology and Immunology. 2013 ; Vol. 57, No. 9. pp. 633-639.
@article{75691df65fb848d5a972787286aff809,
title = "Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection",
abstract = "A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and greater consistency. Administration of 1-methyl-D-tryptophan (1-MT) to inhibit indoleamine 2,3-dioxygenase (IDO) in mice with CCI-induced sepsis increased the survival rate and tended to up-regulate IL-10/IL-12 serum concentrations. The effectiveness of 1-MT was confirmed by increases in IL-10 over IL-12 in bone marrow-derived dendritic cells (BMDCs) treated with LPS and 1-MT and a superior survival rate 24 hr after injection of these double treated BMDCs in the CCI-induced sepsis model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis. The present findings using this newly developed model suggest that inhibition of IDO alleviates the severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic septic inflammation.",
keywords = "Clinical immunology, Dendritic cells, Innate immunity",
author = "Yim, {Hong Soon} and Choi, {Kyung Min} and Byoungjae Kim and Jung, {In Duk} and Park, {Yeong Min} and Kang, {Yoon Kyoo} and Min-Goo Lee",
year = "2013",
month = "9",
day = "1",
doi = "10.1111/1348-0421.12081",
language = "English",
volume = "57",
pages = "633--639",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "9",

}

TY - JOUR

T1 - Effect of 1-methyl-D-tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection

AU - Yim, Hong Soon

AU - Choi, Kyung Min

AU - Kim, Byoungjae

AU - Jung, In Duk

AU - Park, Yeong Min

AU - Kang, Yoon Kyoo

AU - Lee, Min-Goo

PY - 2013/9/1

Y1 - 2013/9/1

N2 - A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and greater consistency. Administration of 1-methyl-D-tryptophan (1-MT) to inhibit indoleamine 2,3-dioxygenase (IDO) in mice with CCI-induced sepsis increased the survival rate and tended to up-regulate IL-10/IL-12 serum concentrations. The effectiveness of 1-MT was confirmed by increases in IL-10 over IL-12 in bone marrow-derived dendritic cells (BMDCs) treated with LPS and 1-MT and a superior survival rate 24 hr after injection of these double treated BMDCs in the CCI-induced sepsis model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis. The present findings using this newly developed model suggest that inhibition of IDO alleviates the severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic septic inflammation.

AB - A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and greater consistency. Administration of 1-methyl-D-tryptophan (1-MT) to inhibit indoleamine 2,3-dioxygenase (IDO) in mice with CCI-induced sepsis increased the survival rate and tended to up-regulate IL-10/IL-12 serum concentrations. The effectiveness of 1-MT was confirmed by increases in IL-10 over IL-12 in bone marrow-derived dendritic cells (BMDCs) treated with LPS and 1-MT and a superior survival rate 24 hr after injection of these double treated BMDCs in the CCI-induced sepsis model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis. The present findings using this newly developed model suggest that inhibition of IDO alleviates the severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic septic inflammation.

KW - Clinical immunology

KW - Dendritic cells

KW - Innate immunity

UR - http://www.scopus.com/inward/record.url?scp=84884693531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884693531&partnerID=8YFLogxK

U2 - 10.1111/1348-0421.12081

DO - 10.1111/1348-0421.12081

M3 - Article

VL - 57

SP - 633

EP - 639

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 9

ER -