Effect of alcohol-free red wine concentrates on cholesterol homeostasis: An in vitro and in vivo study

Dong Hyun Lee, Sung Sik Choi, Bo Bae Kim, Se Young Kim, Bo Sik Kang, Sung Joon Lee, Hyun Jin Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Polyphenolic composition of alcohol-free red wine concentrates (AFRWC) was determined by LC-MS/MS. The concentration of salicylic acid in non-flavonoid class and malvidin in flavonoid class was the highest among all the polyphenols determined in AFRWC. In the in vitro model using HepG2 cells, AFRWC was found to be more effective for the reduction of total cholesterol than lovastatin. For the in vivo model, animals were provided with AFRWC at ∼750 mg of total polyphenols/kg body weight per day by oral administration. The amount of AFRWC was established by extrapolation to be equivalent to 375 ml/day wine consumption, which is ∼2-3 glasses of wine per day for a 60 kg human. Despite a high cholesterol diet, a significant reduction in both total cholesterol and LDL-cholesterol was observed when supplemented with AFRWC, but the increase of HDL-cholesterol was not observed. The expression level of mRNA of some hepatic genes participating in cholesterol biosynthesis, cholesterol esterification was found to be influenced by AFRWC supplementation, whereas reverse cholesterol transport involved with HDL-cholesterol was seldom affected showing discrepancy in the expression of associated genes.

Original languageEnglish
Pages (from-to)1964-1971
Number of pages8
JournalProcess Biochemistry
Volume48
Issue number12
DOIs
Publication statusPublished - 2013 Dec

Keywords

  • Alcohol-free red wine concentrates
  • Cholesterol homeostasis
  • Polyphenol
  • Total cholesterol

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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