Effect of anthocyanins on expression of matrix metalloproteinase-2 in naproxen-induced gastric ulcers

Sun Joong Kim, Young Sam Park, Hyun Dong Paik, Hyo-Ihl Chang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Non-steroidal anti-inflammatory drugs cause gastric ulceration through a number of mechanisms including inhibition of PG synthesis, generation of reactive oxygen species (ROS) and induction of apoptosis. Recently, matrix metalloproteinases (MMP) have been suggested to play a crucial role in these mechanisms. The present study investigated the protective effect of anthocyanins isolated from black rice bran (Heugjinjubyeo) against naproxen-induced gastric mucosal injury in rats. The oral administration of anthocyanins (5, 25 or 50mg/kg body weight) showed significant protection against naproxen (80mg/kg body weight)-induced gastric ulcer and inhibited lipid peroxidation in the gastric mucosa. In addition, pretreatment with anthocyanins resulted in a significant increase in the activities of radical-scavenging enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Also biochemical and zymographic analyses suggested that the administration of anthocyanins gives a significant protection against naproxen-induced gastric antral ulcer through scavenging ROS and regulation of matrix metalloproteinase-2 (MMP-2) activity. The results of intracellular radical activation show that anthocyanins suppress the generation of intracellular ROS and attenuate the suppression of MMP-2 activity by naproxen. These results suggest that anthocyanins extracted from black rice may offer potential remedy of gastric antral ulceration.

Original languageEnglish
Pages (from-to)1792-1801
Number of pages10
JournalBritish Journal of Nutrition
Volume106
Issue number12
DOIs
Publication statusPublished - 2011 Dec 28

Fingerprint

Naproxen
Anthocyanins
Matrix Metalloproteinase 2
Stomach Ulcer
Reactive Oxygen Species
Stomach
Body Weight
Gastrointestinal Agents
Glutathione Peroxidase
Gastric Mucosa
Matrix Metalloproteinases
Catalase
Lipid Peroxidation
Superoxide Dismutase
Oral Administration
Anti-Inflammatory Agents
Apoptosis
Wounds and Injuries
Enzymes

Keywords

  • Anthocyanins
  • Gastric ulcers
  • Matrix metalloproteinase
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Effect of anthocyanins on expression of matrix metalloproteinase-2 in naproxen-induced gastric ulcers. / Kim, Sun Joong; Park, Young Sam; Paik, Hyun Dong; Chang, Hyo-Ihl.

In: British Journal of Nutrition, Vol. 106, No. 12, 28.12.2011, p. 1792-1801.

Research output: Contribution to journalArticle

Kim, Sun Joong ; Park, Young Sam ; Paik, Hyun Dong ; Chang, Hyo-Ihl. / Effect of anthocyanins on expression of matrix metalloproteinase-2 in naproxen-induced gastric ulcers. In: British Journal of Nutrition. 2011 ; Vol. 106, No. 12. pp. 1792-1801.
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