Effect of bilirubin on triglyceride synthesis in streptozotocin-induced diabetic nephropathy

Jianwei Xu, Eun Seong Lee, Seon Ha Baek, Shin Young Ahn, Sejoong Kim, Ki Young Na, Dong Wan Chae, Ho Jun Chin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We aimed to elucidate the effect of bilirubin on dyslipidemia and nephropathy in a diabetes mellitus (DM) type I animal model. Sprague-Dawley rats were separated into control, DM, and bilirubin-treated DM (Bil) groups. The Bil group was injected intraperitoneally with 60 mg/kg bilirubin 3 times per week and hepatoma cells were cultured with bilirubin at a concentration of 0.3 mg/dL. The Bil group showed lower serum creatinine levels 5 weeks after diabetes onset. Bilirubin treatment also decreased the amount of mesangial matrix, lowered the expression of renal collagen IV and transforming growth factor (TGF)-β1, and reduced the level of apoptosis in the kidney, compared to the DM group. These changes were accompanied by decreased tissue levels of hydrogen superoxide and NADPH oxidase subunit proteins. Bilirubin decreased serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), free fatty acids, and triglycerides (TGs), as well as the TG content in the liver tissues. Bilirubin suppressed protein expression of LXRα, SREBP-1, SCD-1, and FAS, factors involved in TG synthesis that were elevated in the livers of DM rats and hepatoma cells under high-glucose conditions. In conclusion, bilirubin attenuates renal dysfunction and dyslipidemia in diabetes by suppressing LXRa and SREBP-1 expression and oxidative stress.

Original languageEnglish
Pages (from-to)S155-S163
JournalJournal of Korean Medical Science
Volume29
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Keywords

  • Bilirubin
  • Diabetes-related complications
  • Fibrosis
  • Transforming growth factor beta
  • Triglyceride

ASJC Scopus subject areas

  • Medicine(all)

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