Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells

Taekhee Jung, John Hwan Lee, Soonjung Park, Yong Jin Kim, Joseph Seo, Hye Eun Shim, Ki Suk Kim, Hyon Seok Jang, Hyung Min Chung, Seong Geun Oh, Sung Hwan Moon, Sun Woong Kang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Differentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II receptors. However, BMPs have a very short half-life and may rapidly lose their bioactivity. Thus, a BMP delivery system is required to take advantage of an osteoinductive effect for osteogenic differentiation. Previously, BMP delivery has been designed and evaluated for osteogenic differentiation, focusing on carriers and sustained release system for delivery of BMPs. The effect of the delivery mode in cell culture plate on osteogenic differentiation potential was not evaluated. Herein, to investigate the effect of delivery mode on osteogenic differentiation of BM-MSCs in this study, we fabricated bottom-up release and top-down release systems for culture plate delivery of BMP-2. And also, we selected Arg-Gly-Asp- (RGD-) conjugated alginate hydrogel for BMP-2 delivery because alginate is able to release BMP-2 in a sustained manner and it is a biocompatible material. After 7 days of culture, the bottom-up release system in culture plate significantly stimulated alkaline phosphate activity of human bone marrow-mesenchymal stem cells. The present study highlights the potential value of the tool in stem cell therapy.

Original languageEnglish
Article number7859184
JournalStem Cells International
Volume2017
DOIs
Publication statusPublished - 2017 Jan 1

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Bone Morphogenetic Protein 2
Stem Cells
Cell- and Tissue-Based Therapy
Stem Cell Factor
Hydrogel
Biocompatible Materials
Mesenchymal Stromal Cells
Human Activities
Half-Life
Regeneration
Cell Culture Techniques
Bone Marrow
Phosphates
alginic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Jung, T., Lee, J. H., Park, S., Kim, Y. J., Seo, J., Shim, H. E., ... Kang, S. W. (2017). Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells. Stem Cells International, 2017, [7859184]. https://doi.org/10.1155/2017/7859184

Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells. / Jung, Taekhee; Lee, John Hwan; Park, Soonjung; Kim, Yong Jin; Seo, Joseph; Shim, Hye Eun; Kim, Ki Suk; Jang, Hyon Seok; Chung, Hyung Min; Oh, Seong Geun; Moon, Sung Hwan; Kang, Sun Woong.

In: Stem Cells International, Vol. 2017, 7859184, 01.01.2017.

Research output: Contribution to journalArticle

Jung, T, Lee, JH, Park, S, Kim, YJ, Seo, J, Shim, HE, Kim, KS, Jang, HS, Chung, HM, Oh, SG, Moon, SH & Kang, SW 2017, 'Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells', Stem Cells International, vol. 2017, 7859184. https://doi.org/10.1155/2017/7859184
Jung, Taekhee ; Lee, John Hwan ; Park, Soonjung ; Kim, Yong Jin ; Seo, Joseph ; Shim, Hye Eun ; Kim, Ki Suk ; Jang, Hyon Seok ; Chung, Hyung Min ; Oh, Seong Geun ; Moon, Sung Hwan ; Kang, Sun Woong. / Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells. In: Stem Cells International. 2017 ; Vol. 2017.
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