Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

Jae Han Ho; Hyun Park Soo; Moo Park Kwon; Cheol Yoon Byung; Tae Sung Kim; Hern Lee Jang

doi:10.1055/s-2002-32567

Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

Jae Han Ho, Hyun Park Soo, Moo Park Kwon, Cheol Yoon Byung, Tae Sung Kim, Hern Lee Jang

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5 Citations (Scopus)

Abstract

The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 ^-4 M H ₂O ₂ did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na ⁺/glucose cotransporter, Na ⁺/Pi cotransporter, and Na ⁺/H ⁺ antiporter in the proximal tubule cells. CA (>10 ^-6 M) prevented H ₂O ₂-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H ₂O ₂-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca ²⁺ uptake. In conclusion, CA, in part, prevented H ₂O ₂-induced inhibition of apical transporter activity via decrease of AA release and Ca ²⁺ uptake in primary cultured renal proximal tubule cells.

Original language	English
Pages (from-to)	483-486
Number of pages	4
Journal	Planta Medica
Volume	68
Issue number	6
DOIs	https://doi.org/10.1055/s-2002-32567
Publication status	Published - 2002 Jul 25
Externally published	Yes

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Keywords

Apical transporter
Arachidonic acid
Ca
Caffeic acid
H O
Kidney

ASJC Scopus subject areas

Plant Science
Drug Discovery
Organic Chemistry
Pharmacology

Cite this

Ho, J. H., Soo, H. P., Kwon, M. P., Byung, C. Y., Kim, T. S., & Jang, H. L. (2002). Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro. Planta Medica, 68(6), 483-486. https://doi.org/10.1055/s-2002-32567

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title = "Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro",

abstract = "The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.",

keywords = "Apical transporter, Arachidonic acid, Ca, Caffeic acid, H O, Kidney",

author = "Ho, {Jae Han} and Soo, {Hyun Park} and Kwon, {Moo Park} and Byung, {Cheol Yoon} and Kim, {Tae Sung} and Jang, {Hern Lee}",

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AU - Ho, Jae Han

AU - Soo, Hyun Park

AU - Kwon, Moo Park

AU - Byung, Cheol Yoon

AU - Kim, Tae Sung

AU - Jang, Hern Lee

PY - 2002/7/25

Y1 - 2002/7/25

N2 - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.

AB - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.

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KW - Arachidonic acid

KW - Ca

KW - Caffeic acid

KW - H O

KW - Kidney

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10.1055/s-2002-32567