Abstract
The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.
| Original language | English |
|---|---|
| Pages (from-to) | 483-486 |
| Number of pages | 4 |
| Journal | Planta Medica |
| Volume | 68 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 Jul 25 |
| Externally published | Yes |
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Keywords
- Apical transporter
- Arachidonic acid
- Ca
- Caffeic acid
- H O
- Kidney
ASJC Scopus subject areas
- Plant Science
- Drug Discovery
- Organic Chemistry
- Pharmacology
Cite this
Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro. / Ho, Jae Han; Soo, Hyun Park; Kwon, Moo Park; Byung, Cheol Yoon; Kim, Tae Sung; Jang, Hern Lee.
In: Planta Medica, Vol. 68, No. 6, 25.07.2002, p. 483-486.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro
AU - Ho, Jae Han
AU - Soo, Hyun Park
AU - Kwon, Moo Park
AU - Byung, Cheol Yoon
AU - Kim, Tae Sung
AU - Jang, Hern Lee
PY - 2002/7/25
Y1 - 2002/7/25
N2 - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.
AB - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.
KW - Apical transporter
KW - Arachidonic acid
KW - Ca
KW - Caffeic acid
KW - H O
KW - Kidney
UR - http://www.scopus.com/inward/record.url?scp=0036066423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036066423&partnerID=8YFLogxK
U2 - 10.1055/s-2002-32567
DO - 10.1055/s-2002-32567
M3 - Article
VL - 68
SP - 483
EP - 486
JO - Planta Medica
JF - Planta Medica
SN - 0032-0943
IS - 6
ER -