Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

Jae Han Ho, Hyun Park Soo, Moo Park Kwon, Cheol Yoon Byung, Tae Sung Kim, Hern Lee Jang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.

Original languageEnglish
Pages (from-to)483-486
Number of pages4
JournalPlanta Medica
Volume68
Issue number6
DOIs
Publication statusPublished - 2002 Jul 25
Externally publishedYes

Fingerprint

Proximal Kidney Tubule
proximal tubules
Oxidative stress
caffeic acid
transporters
Oxidative Stress
oxidative stress
kidneys
arachidonic acid
Arachidonic Acid
cells
uptake mechanisms
Sodium-Hydrogen Antiporter
Lipid Peroxides
peroxides
cell viability
protective effect
mechanism of action
Cell Survival
Cells

Keywords

  • Apical transporter
  • Arachidonic acid
  • Ca
  • Caffeic acid
  • H O
  • Kidney

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro. / Ho, Jae Han; Soo, Hyun Park; Kwon, Moo Park; Byung, Cheol Yoon; Kim, Tae Sung; Jang, Hern Lee.

In: Planta Medica, Vol. 68, No. 6, 25.07.2002, p. 483-486.

Research output: Contribution to journalArticle

Ho, Jae Han ; Soo, Hyun Park ; Kwon, Moo Park ; Byung, Cheol Yoon ; Kim, Tae Sung ; Jang, Hern Lee. / Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro. In: Planta Medica. 2002 ; Vol. 68, No. 6. pp. 483-486.
@article{614f75ac1fe846458734b7e09816eed0,
title = "Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro",
abstract = "The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.",
keywords = "Apical transporter, Arachidonic acid, Ca, Caffeic acid, H O, Kidney",
author = "Ho, {Jae Han} and Soo, {Hyun Park} and Kwon, {Moo Park} and Byung, {Cheol Yoon} and Kim, {Tae Sung} and Jang, {Hern Lee}",
year = "2002",
month = "7",
day = "25",
doi = "10.1055/s-2002-32567",
language = "English",
volume = "68",
pages = "483--486",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
number = "6",

}

TY - JOUR

T1 - Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

AU - Ho, Jae Han

AU - Soo, Hyun Park

AU - Kwon, Moo Park

AU - Byung, Cheol Yoon

AU - Kim, Tae Sung

AU - Jang, Hern Lee

PY - 2002/7/25

Y1 - 2002/7/25

N2 - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.

AB - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 -4 M H 2O 2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na +/glucose cotransporter, Na +/Pi cotransporter, and Na +/H + antiporter in the proximal tubule cells. CA (>10 -6 M) prevented H 2O 2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H 2O 2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca 2+ uptake. In conclusion, CA, in part, prevented H 2O 2-induced inhibition of apical transporter activity via decrease of AA release and Ca 2+ uptake in primary cultured renal proximal tubule cells.

KW - Apical transporter

KW - Arachidonic acid

KW - Ca

KW - Caffeic acid

KW - H O

KW - Kidney

UR - http://www.scopus.com/inward/record.url?scp=0036066423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036066423&partnerID=8YFLogxK

U2 - 10.1055/s-2002-32567

DO - 10.1055/s-2002-32567

M3 - Article

C2 - 12094287

AN - SCOPUS:0036066423

VL - 68

SP - 483

EP - 486

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 6

ER -