Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

Jae Han Ho; Hyun Park Soo; Moo Park Kwon; Cheol Yoon Byung; Sung Kim Tae; Hern Lee Jang

doi:10.1055/s-2002-32567

Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

Jae Han Ho, Hyun Park Soo, Moo Park Kwon, Cheol Yoon Byung, Sung Kim Tae, Hern Lee Jang

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10^-4 M H₂O₂ did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na⁺/glucose cotransporter, Na⁺/Pi cotransporter, and Na⁺/H⁺ antiporter in the proximal tubule cells. CA (>10^-6 M) prevented H₂O₂-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H₂O₂-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca²⁺ uptake. In conclusion, CA, in part, prevented H₂O₂-induced inhibition of apical transporter activity via decrease of AA release and Ca²⁺ uptake in primary cultured renal proximal tubule cells.

Original language	English
Pages (from-to)	483-486
Number of pages	4
Journal	Planta Medica
Volume	68
Issue number	6
DOIs	https://doi.org/10.1055/s-2002-32567
Publication status	Published - 2002
Externally published	Yes

Keywords

Apical transporter
Arachidonic acid
Ca
Caffeic acid
HO
Kidney

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro",

abstract = "The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10-4 M H2O2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na+/glucose cotransporter, Na+/Pi cotransporter, and Na+/H+ antiporter in the proximal tubule cells. CA (>10-6 M) prevented H2O2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H2O2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca2+ uptake. In conclusion, CA, in part, prevented H2O2-induced inhibition of apical transporter activity via decrease of AA release and Ca2+ uptake in primary cultured renal proximal tubule cells.",

keywords = "Apical transporter, Arachidonic acid, Ca, Caffeic acid, HO, Kidney",

author = "Ho, {Jae Han} and Soo, {Hyun Park} and Kwon, {Moo Park} and Byung, {Cheol Yoon} and Tae, {Sung Kim} and Jang, {Hern Lee}",

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T1 - Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro

AU - Ho, Jae Han

AU - Soo, Hyun Park

AU - Kwon, Moo Park

AU - Byung, Cheol Yoon

AU - Tae, Sung Kim

AU - Jang, Hern Lee

PY - 2002

Y1 - 2002

N2 - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10-4 M H2O2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na+/glucose cotransporter, Na+/Pi cotransporter, and Na+/H+ antiporter in the proximal tubule cells. CA (>10-6 M) prevented H2O2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H2O2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca2+ uptake. In conclusion, CA, in part, prevented H2O2-induced inhibition of apical transporter activity via decrease of AA release and Ca2+ uptake in primary cultured renal proximal tubule cells.

AB - The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10-4 M H2O2 did not affect cell viability regardless of incubation time, However, it decreased apical transporters' activity such as Na+/glucose cotransporter, Na+/Pi cotransporter, and Na+/H+ antiporter in the proximal tubule cells. CA (>10-6 M) prevented H2O2-induced inhibition of apical transporters. Thus, we investigated its action mechanism, CA also prevented H2O2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca2+ uptake. In conclusion, CA, in part, prevented H2O2-induced inhibition of apical transporter activity via decrease of AA release and Ca2+ uptake in primary cultured renal proximal tubule cells.

KW - Apical transporter

KW - Arachidonic acid

KW - Ca

KW - Caffeic acid

KW - HO

KW - Kidney

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