Effect of collagen coating on lipid accumulation and differentiation on 3T3-L1 preadipocyte treated with conjugated linoleic acid

Hyun Seuk Moon, Hong Gu Lee, Ho Hyun Song, Yun Jaie Choi, Chong Su Cho

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study is to make use of trans 10,cis 12 CLA (t-CLA) that has potential for proliferation and differentiation to form adipocyte on the collagen-coated surface. Results provided evidences of good adhesion, growth, viability, and differentiation of adipocyte on collagen-coated surface compared with non-coated surface. Also, the results showed that mouse 3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface, and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo full maturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA was higher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. These results suggest that collagen coating may provide a promising approach to develop a new adipocyte replacement strategy using CLA.

Original languageEnglish
Pages (from-to)261-264
Number of pages4
JournalKey Engineering Materials
Volume342-343
DOIs
Publication statusPublished - 2007

Keywords

  • Adipocyte differentiation
  • Collagen
  • Extracellular matrix (ECM)
  • Lipid accumulation
  • Trans10,cis12 conjugated linoleic aicd (t-CLA)

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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