Effect of crude glycerol-derived inhibitors on ethanol production by Enterobacter aerogenes

Sang Jun Lee, Sung Bong Kim, Seong Woo Kang, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


In this study, ethanol production from pure and crude glycerol using Enterobacter aerogenes ATCC 29007 was evaluated under anaerobic culture conditions. Inhibitory effects of substrate concentrations, pH, and salt concentrations were investigated based on crude glycerol components. Ethanol production was performed with pure glycerol concentrations ranging from 5 to 30 g/L to evaluate the effects of substrate concentration and osmotic pressure. The consumed glycerol was 5-14.33 g/L, and the yield of ethanol was higher than 0.75 mol ethanol/mol glycerol after 24 h of cultivation. To evaluate the inhibitory effects of salts (NaCl and KCl), experiments were performed with 0-20 g/L of each salt. Inhibitory effects of salts were strongest at high salt concentrations. The inhibitory effect of pH was performed in the pH range 4-10, and cell growth and ethanol production were highest at pH 5-6. Also, ethanol production was slightly inhibited at low concentration of crude glycerol comparison with pure glycerol. However, significant inhibitory effects were not observed at 1.5 and 2% crude glycerol which showed higher ethanol production compared to pure glycerol.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalBioprocess and Biosystems Engineering
Issue number1-2
Publication statusPublished - 2012 Jan


  • Enterobacter aerogenes
  • Ethanol production
  • Glycerol
  • Inhibitory effect

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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